R-universe search: topic:amplicon

Showing 4 of total 4 results (show query)

bioc

dada2:Accurate, high-resolution sample inference from amplicon sequencing data

The dada2 package infers exact amplicon sequence variants (ASVs) from high-throughput amplicon sequencing data, replacing the coarser and less accurate OTU clustering approach. The dada2 pipeline takes as input demultiplexed fastq files, and outputs the sequence variants and their sample-wise abundances after removing substitution and chimera errors. Taxonomic classification is available via a native implementation of the RDP naive Bayesian classifier, and species-level assignment to 16S rRNA gene fragments by exact matching.

Maintained by Benjamin Callahan. Last updated 5 months ago.

immunooncology microbiome sequencing classification metagenomics amplicon bioconductor bioinformatics metabarcoding taxonomy cpp

487 stars 13.17 score 3.0k scripts 4 dependents

bioc

decontam:Identify Contaminants in Marker-gene and Metagenomics Sequencing Data

Simple statistical identification of contaminating sequence features in marker-gene or metagenomics data. Works on any kind of feature derived from environmental sequencing data (e.g. ASVs, OTUs, taxonomic groups, MAGs,...). Requires DNA quantitation data or sequenced negative control samples.

Maintained by Benjamin Callahan. Last updated 5 months ago.

immunooncology microbiome sequencing classification metagenomics amplicon bioinformatics contamination metabarcoding

153 stars 11.42 score 524 scripts 6 dependents

adrientaudiere

MiscMetabar:Miscellaneous Functions for Metabarcoding Analysis

Facilitate the description, transformation, exploration, and reproducibility of metabarcoding analyses. 'MiscMetabar' is mainly built on top of the 'phyloseq', 'dada2' and 'targets' R packages. It helps to build reproducible and robust bioinformatics pipelines in R. 'MiscMetabar' makes ecological analysis of alpha and beta-diversity easier, more reproducible and more powerful by integrating a large number of tools. Important features are described in Taudière A. (2023) <doi:10.21105/joss.06038>.

Maintained by Adrien Taudière. Last updated 11 days ago.

sequencing microbiome metagenomics clustering classification visualization amplicon amplicon-sequencing biodiversity-informatics ecology illumina metabarcoding ngs-analysis

17 stars 6.44 score 23 scripts

bioc

CellBarcode:Cellular DNA Barcode Analysis toolkit

The package CellBarcode performs Cellular DNA Barcode analysis. It can handle all kinds of DNA barcodes, as long as the barcode is within a single sequencing read and has a pattern that can be matched by a regular expression. \code{CellBarcode} can handle barcodes with flexible lengths, with or without UMI (unique molecular identifier). This tool also can be used for pre-processing some amplicon data such as CRISPR gRNA screening, immune repertoire sequencing, and metagenome data.

Maintained by Wenjie Sun. Last updated 11 days ago.

preprocessing qualitycontrol sequencing crispr amplicon amplicon-sequencing cellular-barcode cpp

1 stars 5.86 score 40 scripts