TmCalculator:Melting Temperature of Nucleic Acid Sequences
This tool is extended from methods in Bio.SeqUtils.MeltingTemp of python. The melting temperature of
nucleic acid sequences can be calculated in three method, the
Wallace rule (Thein & Wallace (1986)
<doi:10.1016/S0140-6736(86)90739-7>), empirical formulas based
on G and C content (Marmur J. (1962)
<doi:10.1016/S0022-2836(62)80066-7>, Schildkraut C. (2010)
<doi:10.1002/bip.360030207>, Wetmur J G (1991)
<doi:10.3109/10409239109114069>, Untergasser,A. (2012)
<doi:10.1093/nar/gks596>, von Ahsen N (2001)
<doi:10.1093/clinchem/47.11.1956>) and nearest neighbor
thermodynamics (Breslauer K J (1986)
<doi:10.1073/pnas.83.11.3746>, Sugimoto N (1996)
<doi:10.1093/nar/24.22.4501>, Allawi H (1998)
<doi:10.1093/nar/26.11.2694>, SantaLucia J (2004)
<doi:10.1146/annurev.biophys.32.110601.141800>, Freier S (1986)
<doi:10.1073/pnas.83.24.9373>, Xia T (1998)
<doi:10.1021/bi9809425>, Chen JL (2012)
<doi:10.1021/bi3002709>, Bommarito S (2000)
<doi:10.1093/nar/28.9.1929>, Turner D H (2010)
<doi:10.1093/nar/gkp892>, Sugimoto N (1995)
<doi:10.1016/S0048-9697(98)00088-6>, Allawi H T (1997)
<doi:10.1021/bi962590c>, Santalucia N (2005)
<doi:10.1093/nar/gki918>), and it can also be corrected with
salt ions and chemical compound (SantaLucia J (1996)
<doi:10.1021/bi951907q>, SantaLucia J(1998)
<doi:10.1073/pnas.95.4.1460>, Owczarzy R (2004)
<doi:10.1021/bi034621r>, Owczarzy R (2008)
<doi:10.1021/bi702363u>).
