R-universe search: needs:ComplexHeatmap

bioc

iSEE:Interactive SummarizedExperiment Explorer

Create an interactive Shiny-based graphical user interface for exploring data stored in SummarizedExperiment objects, including row- and column-level metadata. The interface supports transmission of selections between plots and tables, code tracking, interactive tours, interactive or programmatic initialization, preservation of app state, and extensibility to new panel types via S4 classes. Special attention is given to single-cell data in a SingleCellExperiment object with visualization of dimensionality reduction results.

Maintained by Kevin Rue-Albrecht. Last updated 26 days ago.

cellbasedassays clustering dimensionreduction featureextraction geneexpression gui immunooncology shinyapps singlecell transcription transcriptomics visualization dimension-reduction feature-extraction gene-expression hacktoberfest human-cell-atlas shiny single-cell

225 stars 12.86 score 380 scripts 9 dependents

bioc

CATALYST:Cytometry dATa anALYSis Tools

CATALYST provides tools for preprocessing of and differential discovery in cytometry data such as FACS, CyTOF, and IMC. Preprocessing includes i) normalization using bead standards, ii) single-cell deconvolution, and iii) bead-based compensation. For differential discovery, the package provides a number of convenient functions for data processing (e.g., clustering, dimension reduction), as well as a suite of visualizations for exploratory data analysis and exploration of results from differential abundance (DA) and state (DS) analysis in order to identify differences in composition and expression profiles at the subpopulation-level, respectively.

Maintained by Helena L. Crowell. Last updated 4 months ago.

clustering dataimport differentialexpression experimentaldesign flowcytometry immunooncology massspectrometry normalization preprocessing singlecell software statisticalmethod visualization

67 stars 10.99 score 362 scripts 2 dependents

bioc

EnrichedHeatmap:Making Enriched Heatmaps

Enriched heatmap is a special type of heatmap which visualizes the enrichment of genomic signals on specific target regions. Here we implement enriched heatmap by ComplexHeatmap package. Since this type of heatmap is just a normal heatmap but with some special settings, with the functionality of ComplexHeatmap, it would be much easier to customize the heatmap as well as concatenating to a list of heatmaps to show correspondance between different data sources.

Maintained by Zuguang Gu. Last updated 5 months ago.

software visualization sequencing genomeannotation coverage cpp

190 stars 10.87 score 330 scripts 1 dependents

welch-lab

rliger:Linked Inference of Genomic Experimental Relationships

Uses an extension of nonnegative matrix factorization to identify shared and dataset-specific factors. See Welch J, Kozareva V, et al (2019) <doi:10.1016/j.cell.2019.05.006>, and Liu J, Gao C, Sodicoff J, et al (2020) <doi:10.1038/s41596-020-0391-8> for more details.

Maintained by Yichen Wang. Last updated 3 months ago.

nonnegative-matrix-factorization single-cell openblas cpp

408 stars 10.77 score 334 scripts 1 dependents

bioc

muscat:Multi-sample multi-group scRNA-seq data analysis tools

`muscat` provides various methods and visualization tools for DS analysis in multi-sample, multi-group, multi-(cell-)subpopulation scRNA-seq data, including cell-level mixed models and methods based on aggregated “pseudobulk” data, as well as a flexible simulation platform that mimics both single and multi-sample scRNA-seq data.

Maintained by Helena L. Crowell. Last updated 5 months ago.

immunooncology differentialexpression sequencing singlecell software statisticalmethod visualization

184 stars 10.74 score 686 scripts 1 dependents

bioc

celda:CEllular Latent Dirichlet Allocation

Celda is a suite of Bayesian hierarchical models for clustering single-cell RNA-sequencing (scRNA-seq) data. It is able to perform "bi-clustering" and simultaneously cluster genes into gene modules and cells into cell subpopulations. It also contains DecontX, a novel Bayesian method to computationally estimate and remove RNA contamination in individual cells without empty droplet information. A variety of scRNA-seq data visualization functions is also included.

Maintained by Joshua Campbell. Last updated 1 months ago.

singlecell geneexpression clustering sequencing bayesian immunooncology dataimport cpp openmp

147 stars 10.47 score 256 scripts 2 dependents

stemangiola

tidyHeatmap:A Tidy Implementation of Heatmap

This is a tidy implementation for heatmap. At the moment it is based on the (great) package 'ComplexHeatmap'. The goal of this package is to interface a tidy data frame with this powerful tool. Some of the advantages are: Row and/or columns colour annotations are easy to integrate just specifying one parameter (column names). Custom grouping of rows is easy to specify providing a grouped tbl. For example: df %>% group_by(...). Labels size adjusted by row and column total number. Default use of Brewer and Viridis palettes.

Maintained by Stefano Mangiola. Last updated 2 months ago.

assaydomain infrastructure brewer complexheatmap custom-palette dplyr graphviz heatmap mtcars plotting rstudio scale tibble tidy tidy-data-frame tidybulk tidyverse viridis

335 stars 10.23 score 197 scripts 1 dependents

bioc

singleCellTK:Comprehensive and Interactive Analysis of Single Cell RNA-Seq Data

The Single Cell Toolkit (SCTK) in the singleCellTK package provides an interface to popular tools for importing, quality control, analysis, and visualization of single cell RNA-seq data. SCTK allows users to seamlessly integrate tools from various packages at different stages of the analysis workflow. A general "a la carte" workflow gives users the ability access to multiple methods for data importing, calculation of general QC metrics, doublet detection, ambient RNA estimation and removal, filtering, normalization, batch correction or integration, dimensionality reduction, 2-D embedding, clustering, marker detection, differential expression, cell type labeling, pathway analysis, and data exporting. Curated workflows can be used to run Seurat and Celda. Streamlined quality control can be performed on the command line using the SCTK-QC pipeline. Users can analyze their data using commands in the R console or by using an interactive Shiny Graphical User Interface (GUI). Specific analyses or entire workflows can be summarized and shared with comprehensive HTML reports generated by Rmarkdown. Additional documentation and vignettes can be found at camplab.net/sctk.

Maintained by Joshua David Campbell. Last updated 1 months ago.

singlecell geneexpression differentialexpression alignment clustering immunooncology batcheffect normalization qualitycontrol dataimport gui

182 stars 10.17 score 252 scripts

bioc

diffcyt:Differential discovery in high-dimensional cytometry via high-resolution clustering

Statistical methods for differential discovery analyses in high-dimensional cytometry data (including flow cytometry, mass cytometry or CyTOF, and oligonucleotide-tagged cytometry), based on a combination of high-resolution clustering and empirical Bayes moderated tests adapted from transcriptomics.

Maintained by Lukas M. Weber. Last updated 2 months ago.

immunooncology flowcytometry proteomics singlecell cellbasedassays cellbiology clustering featureextraction software

20 stars 9.98 score 225 scripts 5 dependents

bioc

InteractiveComplexHeatmap:Make Interactive Complex Heatmaps

This package can easily make heatmaps which are produced by the ComplexHeatmap package into interactive applications. It provides two types of interactivities: 1. on the interactive graphics device, and 2. on a Shiny app. It also provides functions for integrating the interactive heatmap widgets for more complex Shiny app development.

Maintained by Zuguang Gu. Last updated 5 months ago.

software visualization sequencing interactive-heatmaps

134 stars 9.52 score 128 scripts 4 dependents

bioc

DEGreport:Report of DEG analysis

Creation of ready-to-share figures of differential expression analyses of count data. It integrates some of the code mentioned in DESeq2 and edgeR vignettes, and report a ranked list of genes according to the fold changes mean and variability for each selected gene.

Maintained by Lorena Pantano. Last updated 5 months ago.

differentialexpression visualization rnaseq reportwriting geneexpression immunooncology bioconductor differential-expression qc report rna-seq smallrna

24 stars 9.42 score 354 scripts 1 dependents

bioc

GeneTonic:Enjoy Analyzing And Integrating The Results From Differential Expression Analysis And Functional Enrichment Analysis

This package provides functionality to combine the existing pieces of the transcriptome data and results, making it easier to generate insightful observations and hypothesis. Its usage is made easy with a Shiny application, combining the benefits of interactivity and reproducibility e.g. by capturing the features and gene sets of interest highlighted during the live session, and creating an HTML report as an artifact where text, code, and output coexist. Using the GeneTonicList as a standardized container for all the required components, it is possible to simplify the generation of multiple visualizations and summaries.

Maintained by Federico Marini. Last updated 3 months ago.

gui geneexpression software transcription transcriptomics visualization differentialexpression pathways reportwriting genesetenrichment annotation go shinyapps bioconductor bioconductor-package data-exploration data-visualization functional-enrichment-analysis gene-expression pathway-analysis reproducible-research rna-seq-analysis rna-seq-data shiny transcriptome user-friendly

77 stars 8.28 score 37 scripts 1 dependents

bioc

POMA:Tools for Omics Data Analysis

The POMA package offers a comprehensive toolkit designed for omics data analysis, streamlining the process from initial visualization to final statistical analysis. Its primary goal is to simplify and unify the various steps involved in omics data processing, making it more accessible and manageable within a single, intuitive R package. Emphasizing on reproducibility and user-friendliness, POMA leverages the standardized SummarizedExperiment class from Bioconductor, ensuring seamless integration and compatibility with a wide array of Bioconductor tools. This approach guarantees maximum flexibility and replicability, making POMA an essential asset for researchers handling omics datasets. See https://github.com/pcastellanoescuder/POMAShiny. Paper: Castellano-Escuder et al. (2021) <doi:10.1371/journal.pcbi.1009148> for more details.

Maintained by Pol Castellano-Escuder. Last updated 4 months ago.

batcheffect classification clustering decisiontree dimensionreduction multidimensionalscaling normalization preprocessing principalcomponent regression rnaseq software statisticalmethod visualization bioconductor bioinformatics data-visualization dimension-reduction exploratory-data-analysis machine-learning omics-data-integration pipeline pre-processing statistical-analysis user-friendly workflow

11 stars 8.16 score 20 scripts 1 dependents

bioc

monaLisa:Binned Motif Enrichment Analysis and Visualization

Useful functions to work with sequence motifs in the analysis of genomics data. These include methods to annotate genomic regions or sequences with predicted motif hits and to identify motifs that drive observed changes in accessibility or expression. Functions to produce informative visualizations of the obtained results are also provided.

Maintained by Michael Stadler. Last updated 8 days ago.

motifannotation visualization featureextraction epigenetics

40 stars 8.10 score 53 scripts

bioc

FLAMES:FLAMES: Full Length Analysis of Mutations and Splicing in long read RNA-seq data

Semi-supervised isoform detection and annotation from both bulk and single-cell long read RNA-seq data. Flames provides automated pipelines for analysing isoforms, as well as intermediate functions for manual execution.

Maintained by Changqing Wang. Last updated 2 days ago.

rnaseq singlecell transcriptomics dataimport differentialsplicing alternativesplicing geneexpression longread zlib curl bzip2 xz-utils cpp

33 stars 8.04 score 12 scripts

bioc

simplifyEnrichment:Simplify Functional Enrichment Results

A new clustering algorithm, "binary cut", for clustering similarity matrices of functional terms is implemeted in this package. It also provides functions for visualizing, summarizing and comparing the clusterings.

Maintained by Zuguang Gu. Last updated 5 months ago.

software visualization go clustering genesetenrichment

113 stars 8.02 score 196 scripts

bioc

hermes:Preprocessing, analyzing, and reporting of RNA-seq data

Provides classes and functions for quality control, filtering, normalization and differential expression analysis of pre-processed `RNA-seq` data. Data can be imported from `SummarizedExperiment` as well as `matrix` objects and can be annotated from `BioMart`. Filtering for genes without too low expression or containing required annotations, as well as filtering for samples with sufficient correlation to other samples or total number of reads is supported. The standard normalization methods including cpm, rpkm and tpm can be used, and 'DESeq2` as well as voom differential expression analyses are available.

Maintained by Daniel Sabanés Bové. Last updated 5 months ago.

rnaseq differentialexpression normalization preprocessing qualitycontrol rna-seq statistical-engineering

11 stars 7.77 score 48 scripts 1 dependents

bioc

BioNERO:Biological Network Reconstruction Omnibus

BioNERO aims to integrate all aspects of biological network inference in a single package, including data preprocessing, exploratory analyses, network inference, and analyses for biological interpretations. BioNERO can be used to infer gene coexpression networks (GCNs) and gene regulatory networks (GRNs) from gene expression data. Additionally, it can be used to explore topological properties of protein-protein interaction (PPI) networks. GCN inference relies on the popular WGCNA algorithm. GRN inference is based on the "wisdom of the crowds" principle, which consists in inferring GRNs with multiple algorithms (here, CLR, GENIE3 and ARACNE) and calculating the average rank for each interaction pair. As all steps of network analyses are included in this package, BioNERO makes users avoid having to learn the syntaxes of several packages and how to communicate between them. Finally, users can also identify consensus modules across independent expression sets and calculate intra and interspecies module preservation statistics between different networks.

Maintained by Fabricio Almeida-Silva. Last updated 5 months ago.

software geneexpression generegulation systemsbiology graphandnetwork preprocessing network networkinference

27 stars 7.69 score 50 scripts 1 dependents

sssydysss

TransProR:Analysis and Visualization of Multi-Omics Data

A tool for comprehensive transcriptomic data analysis, with a focus on transcript-level data preprocessing, expression profiling, differential expression analysis, and functional enrichment. It enables researchers to identify key biological processes, disease biomarkers, and gene regulatory mechanisms. 'TransProR' is aimed at researchers and bioinformaticians working with RNA-Seq data, providing an intuitive framework for in-depth analysis and visualization of transcriptomic datasets. The package includes comprehensive documentation and usage examples to guide users through the entire analysis pipeline. The differential expression analysis methods incorporated in the package include 'limma' (Ritchie et al., 2015, <doi:10.1093/nar/gkv007>; Smyth, 2005, <doi:10.1007/0-387-29362-0_23>), 'edgeR' (Robinson et al., 2010, <doi:10.1093/bioinformatics/btp616>), 'DESeq2' (Love et al., 2014, <doi:10.1186/s13059-014-0550-8>), and Wilcoxon tests (Li et al., 2022, <doi:10.1186/s13059-022-02648-4>), providing flexible and robust approaches to RNA-Seq data analysis. For more information, refer to the package vignettes and related publications.

Maintained by Dongyue Yu. Last updated 1 months ago.

190 stars 7.59 score 34 scripts

bioc

cola:A Framework for Consensus Partitioning

Subgroup classification is a basic task in genomic data analysis, especially for gene expression and DNA methylation data analysis. It can also be used to test the agreement to known clinical annotations, or to test whether there exist significant batch effects. The cola package provides a general framework for subgroup classification by consensus partitioning. It has the following features: 1. It modularizes the consensus partitioning processes that various methods can be easily integrated. 2. It provides rich visualizations for interpreting the results. 3. It allows running multiple methods at the same time and provides functionalities to straightforward compare results. 4. It provides a new method to extract features which are more efficient to separate subgroups. 5. It automatically generates detailed reports for the complete analysis. 6. It allows applying consensus partitioning in a hierarchical manner.

Maintained by Zuguang Gu. Last updated 2 months ago.

clustering geneexpression classification software consensus-clustering cpp

61 stars 7.49 score 112 scripts

bioc

ELMER:Inferring Regulatory Element Landscapes and Transcription Factor Networks Using Cancer Methylomes

ELMER is designed to use DNA methylation and gene expression from a large number of samples to infere regulatory element landscape and transcription factor network in primary tissue.

Maintained by Tiago Chedraoui Silva. Last updated 5 months ago.

dnamethylation geneexpression motifannotation software generegulation transcription network

7.42 score 176 scripts

bioc

PeacoQC:Peak-based selection of high quality cytometry data

This is a package that includes pre-processing and quality control functions that can remove margin events, compensate and transform the data and that will use PeacoQCSignalStability for quality control. This last function will first detect peaks in each channel of the flowframe. It will remove anomalies based on the IsolationTree function and the MAD outlier detection method. This package can be used for both flow- and mass cytometry data.

Maintained by Annelies Emmaneel. Last updated 5 months ago.

flowcytometry qualitycontrol preprocessing peakdetection

16 stars 7.38 score 28 scripts 3 dependents

jokergoo

spiralize:Visualize Data on Spirals

It visualizes data along an Archimedean spiral <https://en.wikipedia.org/wiki/Archimedean_spiral>, makes so-called spiral graph or spiral chart. It has two major advantages for visualization: 1. It is able to visualize data with very long axis with high resolution. 2. It is efficient for time series data to reveal periodic patterns.

Maintained by Zuguang Gu. Last updated 10 months ago.

149 stars 7.37 score 35 scripts 3 dependents

kharchenkolab

conos:Clustering on Network of Samples

Wires together large collections of single-cell RNA-seq datasets, which allows for both the identification of recurrent cell clusters and the propagation of information between datasets in multi-sample or atlas-scale collections. 'Conos' focuses on the uniform mapping of homologous cell types across heterogeneous sample collections. For instance, users could investigate a collection of dozens of peripheral blood samples from cancer patients combined with dozens of controls, which perhaps includes samples of a related tissue such as lymph nodes. This package interacts with data available through the 'conosPanel' package, which is available in a 'drat' repository. To access this data package, see the instructions at <https://github.com/kharchenkolab/conos>. The size of the 'conosPanel' package is approximately 12 MB.

Maintained by Evan Biederstedt. Last updated 1 years ago.

batch-correction scrna-seq single-cell-rna-seq openblas cpp openmp

205 stars 7.33 score 258 scripts

bioc

TBSignatureProfiler:Profile RNA-Seq Data Using TB Pathway Signatures

Gene signatures of TB progression, TB disease, and other TB disease states have been validated and published previously. This package aggregates known signatures and provides computational tools to enlist their usage on other datasets. The TBSignatureProfiler makes it easy to profile RNA-Seq data using these signatures and includes common signature profiling tools including ASSIGN, GSVA, and ssGSEA. Original models for some gene signatures are also available. A shiny app provides some functionality alongside for detailed command line accessibility.

Maintained by Aubrey R. Odom. Last updated 3 months ago.

geneexpression differentialexpression bioconductor-package biomarkers gene-signatures tuberculosis

12 stars 7.25 score 23 scripts

bioc

iSEEu:iSEE Universe

iSEEu (the iSEE universe) contains diverse functionality to extend the usage of the iSEE package, including additional classes for the panels, or modes allowing easy configuration of iSEE applications.

Maintained by Kevin Rue-Albrecht. Last updated 5 months ago.

immunooncology visualization gui dimensionreduction featureextraction clustering transcription geneexpression transcriptomics singlecell cellbasedassays hacktoberfest

9 stars 7.15 score 35 scripts 1 dependents

bioc

DEP:Differential Enrichment analysis of Proteomics data

This package provides an integrated analysis workflow for robust and reproducible analysis of mass spectrometry proteomics data for differential protein expression or differential enrichment. It requires tabular input (e.g. txt files) as generated by quantitative analysis softwares of raw mass spectrometry data, such as MaxQuant or IsobarQuant. Functions are provided for data preparation, filtering, variance normalization and imputation of missing values, as well as statistical testing of differentially enriched / expressed proteins. It also includes tools to check intermediate steps in the workflow, such as normalization and missing values imputation. Finally, visualization tools are provided to explore the results, including heatmap, volcano plot and barplot representations. For scientists with limited experience in R, the package also contains wrapper functions that entail the complete analysis workflow and generate a report. Even easier to use are the interactive Shiny apps that are provided by the package.

Maintained by Arne Smits. Last updated 5 months ago.

immunooncology proteomics massspectrometry differentialexpression datarepresentation

7.10 score 628 scripts

bioc

RiboCrypt:Interactive visualization in genomics

R Package for interactive visualization and browsing NGS data. It contains a browser for both transcript and genomic coordinate view. In addition a QC and general metaplots are included, among others differential translation plots and gene expression plots. The package is still under development.

Maintained by Michal Swirski. Last updated 4 days ago.

software sequencing riboseq rnaseq

5 stars 7.08 score 22 scripts

bioc

sechm:sechm: Complex Heatmaps from a SummarizedExperiment

sechm provides a simple interface between SummarizedExperiment objects and the ComplexHeatmap package. It enables plotting annotated heatmaps from SE objects, with easy access to rowData and colData columns, and implements a number of features to make the generation of heatmaps easier and more flexible. These functionalities used to be part of the SEtools package.

Maintained by Pierre-Luc Germain. Last updated 1 months ago.

geneexpression visualization

6 stars 7.03 score 60 scripts 2 dependents

bioc

pipeComp:pipeComp pipeline benchmarking framework

A simple framework to facilitate the comparison of pipelines involving various steps and parameters. The `pipelineDefinition` class represents pipelines as, minimally, a set of functions consecutively executed on the output of the previous one, and optionally accompanied by step-wise evaluation and aggregation functions. Given such an object, a set of alternative parameters/methods, and benchmark datasets, the `runPipeline` function then proceeds through all combinations arguments, avoiding recomputing the same step twice and compiling evaluations on the fly to avoid storing potentially large intermediate data.

Maintained by Pierre-Luc Germain. Last updated 5 months ago.

geneexpression transcriptomics clustering datarepresentation benchmark bioconductor pipeline-benchmarking pipelines single-cell-rna-seq

41 stars 7.02 score 43 scripts

bioc

COCOA:Coordinate Covariation Analysis

COCOA is a method for understanding epigenetic variation among samples. COCOA can be used with epigenetic data that includes genomic coordinates and an epigenetic signal, such as DNA methylation and chromatin accessibility data. To describe the method on a high level, COCOA quantifies inter-sample variation with either a supervised or unsupervised technique then uses a database of "region sets" to annotate the variation among samples. A region set is a set of genomic regions that share a biological annotation, for instance transcription factor (TF) binding regions, histone modification regions, or open chromatin regions. COCOA can identify region sets that are associated with epigenetic variation between samples and increase understanding of variation in your data.

Maintained by John Lawson. Last updated 5 months ago.

epigenetics dnamethylation atacseq dnaseseq methylseq methylationarray principalcomponent genomicvariation generegulation genomeannotation systemsbiology functionalgenomics chipseq sequencing immunooncology dna-methylation pca

10 stars 7.02 score 21 scripts

bioc

GenomicSuperSignature:Interpretation of RNA-seq experiments through robust, efficient comparison to public databases

This package provides a novel method for interpreting new transcriptomic datasets through near-instantaneous comparison to public archives without high-performance computing requirements. Through the pre-computed index, users can identify public resources associated with their dataset such as gene sets, MeSH term, and publication. Functions to identify interpretable annotations and intuitive visualization options are implemented in this package.

Maintained by Sehyun Oh. Last updated 5 months ago.

transcriptomics systemsbiology principalcomponent rnaseq sequencing pathways clustering bioconductor-package exploratory-data-analysis gsea mesh principal-component-analysis rna-sequencing-profiles transferlearning u24ca289073

16 stars 6.97 score 59 scripts

bioc

musicatk:Mutational Signature Comprehensive Analysis Toolkit

Mutational signatures are carcinogenic exposures or aberrant cellular processes that can cause alterations to the genome. We created musicatk (MUtational SIgnature Comprehensive Analysis ToolKit) to address shortcomings in versatility and ease of use in other pre-existing computational tools. Although many different types of mutational data have been generated, current software packages do not have a flexible framework to allow users to mix and match different types of mutations in the mutational signature inference process. Musicatk enables users to count and combine multiple mutation types, including SBS, DBS, and indels. Musicatk calculates replication strand, transcription strand and combinations of these features along with discovery from unique and proprietary genomic feature associated with any mutation type. Musicatk also implements several methods for discovery of new signatures as well as methods to infer exposure given an existing set of signatures. Musicatk provides functions for visualization and downstream exploratory analysis including the ability to compare signatures between cohorts and find matching signatures in COSMIC V2 or COSMIC V3.

Maintained by Joshua D. Campbell. Last updated 5 months ago.

software biologicalquestion somaticmutation variantannotation

13 stars 6.97 score 20 scripts

bioc

isomiRs:Analyze isomiRs and miRNAs from small RNA-seq

Characterization of miRNAs and isomiRs, clustering and differential expression.

Maintained by Lorena Pantano. Last updated 5 months ago.

mirna rnaseq differentialexpression clustering immunooncology analyze-isomirs bioconductor isomirs

8 stars 6.97 score 43 scripts

bioc

treeclimbR:An algorithm to find optimal signal levels in a tree

The arrangement of hypotheses in a hierarchical structure appears in many research fields and often indicates different resolutions at which data can be viewed. This raises the question of which resolution level the signal should best be interpreted on. treeclimbR provides a flexible method to select optimal resolution levels (potentially different levels in different parts of the tree), rather than cutting the tree at an arbitrary level. treeclimbR uses a tuning parameter to generate candidate resolutions and from these selects the optimal one.

Maintained by Charlotte Soneson. Last updated 3 months ago.

statisticalmethod cellbasedassays

20 stars 6.86 score 45 scripts

bioc

COTAN:COexpression Tables ANalysis

Statistical and computational method to analyze the co-expression of gene pairs at single cell level. It provides the foundation for single-cell gene interactome analysis. The basic idea is studying the zero UMI counts' distribution instead of focusing on positive counts; this is done with a generalized contingency tables framework. COTAN can effectively assess the correlated or anti-correlated expression of gene pairs. It provides a numerical index related to the correlation and an approximate p-value for the associated independence test. COTAN can also evaluate whether single genes are differentially expressed, scoring them with a newly defined global differentiation index. Moreover, this approach provides ways to plot and cluster genes according to their co-expression pattern with other genes, effectively helping the study of gene interactions and becoming a new tool to identify cell-identity marker genes.

Maintained by Galfrè Silvia Giulia. Last updated 20 days ago.

systemsbiology transcriptomics geneexpression singlecell

16 stars 6.85 score 96 scripts

junjunlab

ClusterGVis:One-Step to Cluster and Visualize Gene Expression Data

Streamlining the clustering and visualization of time-series gene expression data from RNA-Seq experiments, this tool supports fuzzy c-means and k-means clustering algorithms. It is compatible with outputs from widely-used packages such as 'Seurat', 'Monocle', and 'WGCNA', enabling seamless downstream visualization and analysis. See Lokesh Kumar and Matthias E Futschik (2007) <doi:10.6026/97320630002005> for more details.

Maintained by Jun Zhang. Last updated 23 days ago.

sequencing clusterprofiler summarizedexperiment mfuzz complexheatmap gene-clustering gene-expression visualization

281 stars 6.80 score 30 scripts

jokergoo

pkgndep:Analyze Dependency Heaviness of R Packages

A new metric named 'dependency heaviness' is proposed that measures the number of additional dependency packages that a parent package brings to its child package and are unique to the dependency packages imported by all other parents. The dependency heaviness analysis is visualized by a customized heatmap. The package is described in <doi:10.1093/bioinformatics/btac449>. We have also performed the dependency heaviness analysis on the CRAN/Bioconductor package ecosystem and the results are implemented as a web-based database which provides comprehensive tools for querying dependencies of individual R packages. The systematic analysis on the CRAN/Bioconductor ecosystem is described in <doi:10.1016/j.jss.2023.111610>. From 'pkgndep' version 2.0.0, the heaviness database includes snapshots of the CRAN/Bioconductor ecosystems for many old R versions.

Maintained by Zuguang Gu. Last updated 29 days ago.

47 stars 6.75 score 30 scripts

bioc

CytoPipeline:Automation and visualization of flow cytometry data analysis pipelines

This package provides support for automation and visualization of flow cytometry data analysis pipelines. In the current state, the package focuses on the preprocessing and quality control part. The framework is based on two main S4 classes, i.e. CytoPipeline and CytoProcessingStep. The pipeline steps are linked to corresponding R functions - that are either provided in the CytoPipeline package itself, or exported from a third party package, or coded by the user her/himself. The processing steps need to be specified centrally and explicitly using either a json input file or through step by step creation of a CytoPipeline object with dedicated methods. After having run the pipeline, obtained results at all steps can be retrieved and visualized thanks to file caching (the running facility uses a BiocFileCache implementation). The package provides also specific visualization tools like pipeline workflow summary display, and 1D/2D comparison plots of obtained flowFrames at various steps of the pipeline.

Maintained by Philippe Hauchamps. Last updated 5 months ago.

flowcytometry preprocessing qualitycontrol workflowstep immunooncology software visualization

4 stars 6.71 score 18 scripts 2 dependents

bioc

SPONGE:Sparse Partial Correlations On Gene Expression

This package provides methods to efficiently detect competitive endogeneous RNA interactions between two genes. Such interactions are mediated by one or several miRNAs such that both gene and miRNA expression data for a larger number of samples is needed as input. The SPONGE package now also includes spongEffects: ceRNA modules offer patient-specific insights into the miRNA regulatory landscape.

Maintained by Markus List. Last updated 5 months ago.

geneexpression transcription generegulation networkinference transcriptomics systemsbiology regression randomforest machinelearning

6.66 score 38 scripts 1 dependents

bioc

ViSEAGO:ViSEAGO: a Bioconductor package for clustering biological functions using Gene Ontology and semantic similarity

The main objective of ViSEAGO package is to carry out a data mining of biological functions and establish links between genes involved in the study. We developed ViSEAGO in R to facilitate functional Gene Ontology (GO) analysis of complex experimental design with multiple comparisons of interest. It allows to study large-scale datasets together and visualize GO profiles to capture biological knowledge. The acronym stands for three major concepts of the analysis: Visualization, Semantic similarity and Enrichment Analysis of Gene Ontology. It provides access to the last current GO annotations, which are retrieved from one of NCBI EntrezGene, Ensembl or Uniprot databases for several species. Using available R packages and novel developments, ViSEAGO extends classical functional GO analysis to focus on functional coherence by aggregating closely related biological themes while studying multiple datasets at once. It provides both a synthetic and detailed view using interactive functionalities respecting the GO graph structure and ensuring functional coherence supplied by semantic similarity. ViSEAGO has been successfully applied on several datasets from different species with a variety of biological questions. Results can be easily shared between bioinformaticians and biologists, enhancing reporting capabilities while maintaining reproducibility.

Maintained by Aurelien Brionne. Last updated 3 months ago.

software annotation go genesetenrichment multiplecomparison clustering visualization

6.64 score 22 scripts

bioc

simona:Semantic Similarity on Bio-Ontologies

This package implements infrastructures for ontology analysis by offering efficient data structures, fast ontology traversal methods, and elegant visualizations. It provides a robust toolbox supporting over 70 methods for semantic similarity analysis.

Maintained by Zuguang Gu. Last updated 5 months ago.

software annotation go biomedicalinformatics cpp

17 stars 6.62 score 27 scripts 1 dependents

bioc

pathlinkR:Analyze and interpret RNA-Seq results

pathlinkR is an R package designed to facilitate analysis of RNA-Seq results. Specifically, our aim with pathlinkR was to provide a number of tools which take a list of DE genes and perform different analyses on them, aiding with the interpretation of results. Functions are included to perform pathway enrichment, with muliplte databases supported, and tools for visualizing these results. Genes can also be used to create and plot protein-protein interaction networks, all from inside of R.

Maintained by Travis Blimkie. Last updated 3 months ago.

genesetenrichment network pathways reactome rnaseq networkenrichment bioinformatics networks pathway-enrichment-analysis visualization

28 stars 6.59 score 2 scripts

bioc

nipalsMCIA:Multiple Co-Inertia Analysis via the NIPALS Method

Computes Multiple Co-Inertia Analysis (MCIA), a dimensionality reduction (jDR) algorithm, for a multi-block dataset using a modification to the Nonlinear Iterative Partial Least Squares method (NIPALS) proposed in (Hanafi et. al, 2010). Allows multiple options for row- and table-level preprocessing, and speeds up computation of variance explained. Vignettes detail application to bulk- and single cell- multi-omics studies.

Maintained by Maximilian Mattessich. Last updated 1 months ago.

software clustering classification multiplecomparison normalization preprocessing singlecell

6 stars 6.51 score 10 scripts

bioc

Statial:A package to identify changes in cell state relative to spatial associations

Statial is a suite of functions for identifying changes in cell state. The functionality provided by Statial provides robust quantification of cell type localisation which are invariant to changes in tissue structure. In addition to this Statial uncovers changes in marker expression associated with varying levels of localisation. These features can be used to explore how the structure and function of different cell types may be altered by the agents they are surrounded with.

Maintained by Farhan Ameen. Last updated 5 months ago.

singlecell spatial classification single-cell

5 stars 6.49 score 23 scripts

bioc

Xeva:Analysis of patient-derived xenograft (PDX) data

The Xeva package provides efficient and powerful functions for patient-drived xenograft (PDX) based pharmacogenomic data analysis. This package contains a set of functions to perform analysis of patient-derived xenograft data. This package was developed by the BHKLab, for further information please see our documentation.

Maintained by Benjamin Haibe-Kains. Last updated 12 days ago.

geneexpression pharmacogenetics pharmacogenomics software classification

11 stars 6.48 score 17 scripts

bioc

Moonlight2R:Identify oncogenes and tumor suppressor genes from omics data

The understanding of cancer mechanism requires the identification of genes playing a role in the development of the pathology and the characterization of their role (notably oncogenes and tumor suppressors). We present an updated version of the R/bioconductor package called MoonlightR, namely Moonlight2R, which returns a list of candidate driver genes for specific cancer types on the basis of omics data integration. The Moonlight framework contains a primary layer where gene expression data and information about biological processes are integrated to predict genes called oncogenic mediators, divided into putative tumor suppressors and putative oncogenes. This is done through functional enrichment analyses, gene regulatory networks and upstream regulator analyses to score the importance of well-known biological processes with respect to the studied cancer type. By evaluating the effect of the oncogenic mediators on biological processes or through random forests, the primary layer predicts two putative roles for the oncogenic mediators: i) tumor suppressor genes (TSGs) and ii) oncogenes (OCGs). As gene expression data alone is not enough to explain the deregulation of the genes, a second layer of evidence is needed. We have automated the integration of a secondary mutational layer through new functionalities in Moonlight2R. These functionalities analyze mutations in the cancer cohort and classifies these into driver and passenger mutations using the driver mutation prediction tool, CScape-somatic. Those oncogenic mediators with at least one driver mutation are retained as the driver genes. As a consequence, this methodology does not only identify genes playing a dual role (e.g. TSG in one cancer type and OCG in another) but also helps in elucidating the biological processes underlying their specific roles. In particular, Moonlight2R can be used to discover OCGs and TSGs in the same cancer type. This may for instance help in answering the question whether some genes change role between early stages (I, II) and late stages (III, IV). In the future, this analysis could be useful to determine the causes of different resistances to chemotherapeutic treatments. An additional mechanistic layer evaluates if there are mutations affecting the protein stability of the transcription factors (TFs) of the TSGs and OCGs, as that may have an effect on the expression of the genes.

Maintained by Matteo Tiberti. Last updated 2 months ago.

dnamethylation differentialmethylation generegulation geneexpression methylationarray differentialexpression pathways network survival genesetenrichment networkenrichment

5 stars 6.41 score 43 scripts

bioc

YAPSA:Yet Another Package for Signature Analysis

This package provides functions and routines for supervised analyses of mutational signatures (i.e., the signatures have to be known, cf. L. Alexandrov et al., Nature 2013 and L. Alexandrov et al., Bioaxiv 2018). In particular, the family of functions LCD (LCD = linear combination decomposition) can use optimal signature-specific cutoffs which takes care of different detectability of the different signatures. Moreover, the package provides different sets of mutational signatures, including the COSMIC and PCAWG SNV signatures and the PCAWG Indel signatures; the latter infering that with YAPSA, the concept of supervised analysis of mutational signatures is extended to Indel signatures. YAPSA also provides confidence intervals as computed by profile likelihoods and can perform signature analysis on a stratified mutational catalogue (SMC = stratify mutational catalogue) in order to analyze enrichment and depletion patterns for the signatures in different strata.

Maintained by Zuguang Gu. Last updated 5 months ago.

sequencing dnaseq somaticmutation visualization clustering genomicvariation statisticalmethod biologicalquestion

6.41 score 57 scripts

bioc

iSEEtree:Interactive visualisation for microbiome data

iSEEtree is an extension of iSEE for the TreeSummarizedExperiment data container. It provides interactive panel designs to explore hierarchical datasets, such as the microbiome and cell lines.

Maintained by Giulio Benedetti. Last updated 13 days ago.

software visualization microbiome gui shinyapps dataimport shiny-apps visualisation

3 stars 6.28 score 5 scripts

bioc

signifinder:Collection and implementation of public transcriptional cancer signatures

signifinder is an R package for computing and exploring a compendium of tumor signatures. It allows to compute a variety of signatures, based on gene expression values, and return single-sample scores. Currently, signifinder contains more than 60 distinct signatures collected from the literature, relating to multiple tumors and multiple cancer processes.

Maintained by Stefania Pirrotta. Last updated 3 months ago.

geneexpression genetarget immunooncology biomedicalinformatics rnaseq microarray reportwriting visualization singlecell spatial genesignaling

7 stars 6.28 score 15 scripts

david-barnett

microViz:Microbiome Data Analysis and Visualization

Microbiome data visualization and statistics tools built upon phyloseq.

Maintained by David Barnett. Last updated 4 months ago.

microbiome microbiome-analysis microbiota

114 stars 6.22 score 480 scripts

bioc

dominoSignal:Cell Communication Analysis for Single Cell RNA Sequencing

dominoSignal is a package developed to analyze cell signaling through ligand - receptor - transcription factor networks in scRNAseq data. It takes as input information transcriptomic data, requiring counts, z-scored counts, and cluster labels, as well as information on transcription factor activation (such as from SCENIC) and a database of ligand and receptor pairings (such as from CellPhoneDB). This package creates an object storing ligand - receptor - transcription factor linkages by cluster and provides several methods for exploring, summarizing, and visualizing the analysis.

Maintained by Jacob T Mitchell. Last updated 15 days ago.

systemsbiology singlecell transcriptomics network

5 stars 6.20 score 5 scripts

bioc

MOMA:Multi Omic Master Regulator Analysis

This package implements the inference of candidate master regulator proteins from multi-omics' data (MOMA) algorithm, as well as ancillary analysis and visualization functions.

Maintained by Sunny Jones. Last updated 5 months ago.

software networkenrichment networkinference network featureextraction clustering functionalgenomics transcriptomics systemsbiology

6 stars 6.19 score 13 scripts

bioc

CRISPRball:Shiny Application for Interactive CRISPR Screen Visualization, Exploration, Comparison, and Filtering

A Shiny application for visualization, exploration, comparison, and filtering of CRISPR screens analyzed with MAGeCK RRA or MLE. Features include interactive plots with on-click labeling, full customization of plot aesthetics, data upload and/or download, and much more. Quickly and easily explore your CRISPR screen results and generate publication-quality figures in seconds.

Maintained by Jared Andrews. Last updated 3 months ago.

software shinyapps crispr qualitycontrol visualization gui crispr-screen data-visualization interactive-visualizations mageck plotly screening shiny

9 stars 6.03 score 24 scripts

bioc

dar:Differential Abundance Analysis by Consensus

Differential abundance testing in microbiome data challenges both parametric and non-parametric statistical methods, due to its sparsity, high variability and compositional nature. Microbiome-specific statistical methods often assume classical distribution models or take into account compositional specifics. These produce results that range within the specificity vs sensitivity space in such a way that type I and type II error that are difficult to ascertain in real microbiome data when a single method is used. Recently, a consensus approach based on multiple differential abundance (DA) methods was recently suggested in order to increase robustness. With dar, you can use dplyr-like pipeable sequences of DA methods and then apply different consensus strategies. In this way we can obtain more reliable results in a fast, consistent and reproducible way.

Maintained by Francesc Catala-Moll. Last updated 17 days ago.

software sequencing microbiome metagenomics multiplecomparison normalization bioconductor biomarker-discovery differential-abundance-analysis feature-selection microbiology phyloseq

2 stars 5.98 score 8 scripts

bioc

PathoStat:PathoStat Statistical Microbiome Analysis Package

The purpose of this package is to perform Statistical Microbiome Analysis on metagenomics results from sequencing data samples. In particular, it supports analyses on the PathoScope generated report files. PathoStat provides various functionalities including Relative Abundance charts, Diversity estimates and plots, tests of Differential Abundance, Time Series visualization, and Core OTU analysis.

Maintained by Solaiappan Manimaran. Last updated 5 months ago.

microbiome metagenomics graphandnetwork microarray patternlogic principalcomponent sequencing software visualization rnaseq immunooncology

8 stars 5.90 score 8 scripts

bioc

miRspongeR:Identification and analysis of miRNA sponge regulation

This package provides several functions to explore miRNA sponge (also called ceRNA or miRNA decoy) regulation from putative miRNA-target interactions or/and transcriptomics data (including bulk, single-cell and spatial gene expression data). It provides eight popular methods for identifying miRNA sponge interactions, and an integrative method to integrate miRNA sponge interactions from different methods, as well as the functions to validate miRNA sponge interactions, and infer miRNA sponge modules, conduct enrichment analysis of miRNA sponge modules, and conduct survival analysis of miRNA sponge modules. By using a sample control variable strategy, it provides a function to infer sample-specific miRNA sponge interactions. In terms of sample-specific miRNA sponge interactions, it implements three similarity methods to construct sample-sample correlation network.

Maintained by Junpeng Zhang. Last updated 5 months ago.

geneexpression biomedicalinformatics networkenrichment survival microarray software singlecell spatial rnaseq cerna mirna sponge

5 stars 5.88 score 8 scripts

bioc

CCPlotR:Plots For Visualising Cell-Cell Interactions

CCPlotR is an R package for visualising results from tools that predict cell-cell interactions from single-cell RNA-seq data. These plots are generic and can be used to visualise results from multiple tools such as Liana, CellPhoneDB, NATMI etc.

Maintained by Sarah Ennis. Last updated 5 months ago.

singlecell network visualization cellbiology systemsbiology

43 stars 5.81 score 7 scripts

bioc

BindingSiteFinder:Binding site defintion based on iCLIP data

Precise knowledge on the binding sites of an RNA-binding protein (RBP) is key to understand (post-) transcriptional regulatory processes. Here we present a workflow that describes how exact binding sites can be defined from iCLIP data. The package provides functions for binding site definition and result visualization. For details please see the vignette.

Maintained by Mirko Brüggemann. Last updated 13 days ago.

sequencing geneexpression generegulation functionalgenomics coverage dataimport binding-site-classification binding-sites bioconductor-package iclip rna-binding-proteins

6 stars 5.80 score 3 scripts

bioc

GenomicPlot:Plot profiles of next generation sequencing data in genomic features

Visualization of next generation sequencing (NGS) data is essential for interpreting high-throughput genomics experiment results. 'GenomicPlot' facilitates plotting of NGS data in various formats (bam, bed, wig and bigwig); both coverage and enrichment over input can be computed and displayed with respect to genomic features (such as UTR, CDS, enhancer), and user defined genomic loci or regions. Statistical tests on signal intensity within user defined regions of interest can be performed and represented as boxplots or bar graphs. Parallel processing is used to speed up computation on multicore platforms. In addition to genomic plots which is suitable for displaying of coverage of genomic DNA (such as ChIPseq data), metagenomic (without introns) plots can also be made for RNAseq or CLIPseq data as well.

Maintained by Shuye Pu. Last updated 2 months ago.

alternativesplicing chipseq coverage geneexpression rnaseq sequencing software transcription visualization annotation

5 stars 5.78 score 4 scripts

bioc

scRNAseqApp:A single-cell RNAseq Shiny app-package

The scRNAseqApp is a Shiny app package designed for interactive visualization of single-cell data. It is an enhanced version derived from the ShinyCell, repackaged to accommodate multiple datasets. The app enables users to visualize data containing various types of information simultaneously, facilitating comprehensive analysis. Additionally, it includes a user management system to regulate database accessibility for different users.

Maintained by Jianhong Ou. Last updated 19 days ago.

visualization singlecell rnaseq interactive-visualizations multiple-users shiny-apps single-cell-rna-seq

4 stars 5.76 score 3 scripts

bioc

sparrow:Take command of set enrichment analyses through a unified interface

Provides a unified interface to a variety of GSEA techniques from different bioconductor packages. Results are harmonized into a single object and can be interrogated uniformly for quick exploration and interpretation of results. Interactive exploration of GSEA results is enabled through a shiny app provided by a sparrow.shiny sibling package.

Maintained by Steve Lianoglou. Last updated 15 days ago.

genesetenrichment pathways bioinformatics gsea

21 stars 5.74 score 13 scripts

bioc

CaMutQC:An R Package for Comprehensive Filtration and Selection of Cancer Somatic Mutations

CaMutQC is able to filter false positive mutations generated due to technical issues, as well as to select candidate cancer mutations through a series of well-structured functions by labeling mutations with various flags. And a detailed and vivid filter report will be offered after completing a whole filtration or selection section. Also, CaMutQC integrates serveral methods and gene panels for Tumor Mutational Burden (TMB) estimation.

Maintained by Xin Wang. Last updated 5 months ago.

software qualitycontrol genetarget cancer-genomics somatic-mutations

7 stars 5.72 score 1 scripts

bioc

iSEEindex:iSEE extension for a landing page to a custom collection of data sets

This package provides an interface to any collection of data sets within a single iSEE web-application. The main functionality of this package is to define a custom landing page allowing app maintainers to list a custom collection of data sets that users can selected from and directly load objects into an iSEE web-application.

Maintained by Kevin Rue-Albrecht. Last updated 5 months ago.

software infrastructure bioconductor hacktoberfest

2 stars 5.65 score 8 scripts

thermostats

RVA:RNAseq Visualization Automation

Automate downstream visualization & pathway analysis in RNAseq analysis. 'RVA' is a collection of functions that efficiently visualize RNAseq differential expression analysis result from summary statistics tables. It also utilize the Fisher's exact test to evaluate gene set or pathway enrichment in a convenient and efficient manner.

Maintained by Xingpeng Li. Last updated 3 years ago.

9 stars 5.65 score 6 scripts

bioc

SEtools:SEtools: tools for working with SummarizedExperiment

This includes a set of convenience functions for working with the SummarizedExperiment class. Note that plotting functions historically in this package have been moved to the sechm package (see vignette for details).

Maintained by Pierre-Luc Germain. Last updated 5 months ago.

geneexpression

2 stars 5.64 score 72 scripts

bioc

TMSig:Tools for Molecular Signatures

The TMSig package contains tools to prepare, analyze, and visualize named lists of sets, with an emphasis on molecular signatures (such as gene or kinase sets). It includes fast, memory efficient functions to construct sparse incidence and similarity matrices and filter, cluster, invert, and decompose sets. Additionally, bubble heatmaps can be created to visualize the results of any differential or molecular signatures analysis.

Maintained by Tyler Sagendorf. Last updated 5 months ago.

clustering genesetenrichment graphandnetwork pathways visualization gene-sets molecular-signatures

4 stars 5.58 score 4 scripts

bioc

chevreulPlot:Plots used in the chevreulPlot package

Tools for plotting SingleCellExperiment objects in the chevreulPlot package. Includes functions for analysis and visualization of single-cell data. Supported by NIH grants R01CA137124 and R01EY026661 to David Cobrinik.

Maintained by Kevin Stachelek. Last updated 1 months ago.

coverage rnaseq sequencing visualization geneexpression transcription singlecell transcriptomics normalization preprocessing qualitycontrol dimensionreduction dataimport

5.56 score 2 scripts 1 dependents

bioc

iSEEhub:iSEE for the Bioconductor ExperimentHub

This package defines a custom landing page for an iSEE app interfacing with the Bioconductor ExperimentHub. The landing page allows users to browse the ExperimentHub, select a data set, download and cache it, and import it directly into a Bioconductor iSEE app.

Maintained by Kevin Rue-Albrecht. Last updated 5 months ago.

dataimport immunooncology infrastructure shinyapps singlecell software bioconductor bioconductor-package hacktoberfest isee

3 stars 5.56 score 4 scripts

insightsengineering

teal.modules.hermes:RNA-Seq Analysis Modules to Add to a Teal Application

RNA-seq analysis teal modules based on the `hermes` package.

Maintained by Daniel Sabanés Bové. Last updated 1 years ago.

modules rna-seq-analysis shiny

7 stars 5.54 score 32 scripts

bioc

GRaNIE:GRaNIE: Reconstruction cell type specific gene regulatory networks including enhancers using single-cell or bulk chromatin accessibility and RNA-seq data

Genetic variants associated with diseases often affect non-coding regions, thus likely having a regulatory role. To understand the effects of genetic variants in these regulatory regions, identifying genes that are modulated by specific regulatory elements (REs) is crucial. The effect of gene regulatory elements, such as enhancers, is often cell-type specific, likely because the combinations of transcription factors (TFs) that are regulating a given enhancer have cell-type specific activity. This TF activity can be quantified with existing tools such as diffTF and captures differences in binding of a TF in open chromatin regions. Collectively, this forms a gene regulatory network (GRN) with cell-type and data-specific TF-RE and RE-gene links. Here, we reconstruct such a GRN using single-cell or bulk RNAseq and open chromatin (e.g., using ATACseq or ChIPseq for open chromatin marks) and optionally (Capture) Hi-C data. Our network contains different types of links, connecting TFs to regulatory elements, the latter of which is connected to genes in the vicinity or within the same chromatin domain (TAD). We use a statistical framework to assign empirical FDRs and weights to all links using a permutation-based approach.

Maintained by Christian Arnold. Last updated 5 months ago.

software geneexpression generegulation networkinference genesetenrichment biomedicalinformatics genetics transcriptomics atacseq rnaseq graphandnetwork regression transcription chipseq

5.40 score 24 scripts

bioc

iSEEde:iSEE extension for panels related to differential expression analysis

This package contains diverse functionality to extend the usage of the iSEE package, including additional classes for the panels or modes facilitating the analysis of differential expression results. This package does not perform differential expression. Instead, it provides methods to embed precomputed differential expression results in a SummarizedExperiment object, in a manner that is compatible with interactive visualisation in iSEE applications.

Maintained by Kevin Rue-Albrecht. Last updated 5 months ago.

software infrastructure differentialexpression bioconductor hacktoberfest iseeu

1 stars 5.38 score 15 scripts

bioc

iSEEhex:iSEE extension for summarising data points in hexagonal bins

This package provides panels summarising data points in hexagonal bins for `iSEE`. It is part of `iSEEu`, the iSEE universe of panels that extend the `iSEE` package.

Maintained by Kevin Rue-Albrecht. Last updated 5 months ago.

software infrastructure bioconductor iseeu shiny-r

5.38 score 7 scripts 2 dependents

bioc

diffUTR:diffUTR: Streamlining differential exon and 3' UTR usage

The diffUTR package provides a uniform interface and plotting functions for limma/edgeR/DEXSeq -powered differential bin/exon usage. It includes in addition an improved version of the limma::diffSplice method. Most importantly, diffUTR further extends the application of these frameworks to differential UTR usage analysis using poly-A site databases.

Maintained by Pierre-Luc Germain. Last updated 5 months ago.

geneexpression

6 stars 5.38 score 9 scripts

bioc

GeDi:Defining and visualizing the distances between different genesets

The package provides different distances measurements to calculate the difference between genesets. Based on these scores the genesets are clustered and visualized as graph. This is all presented in an interactive Shiny application for easy usage.

Maintained by Annekathrin Nedwed. Last updated 5 months ago.

gui genesetenrichment software transcription rnaseq visualization clustering pathways reportwriting go kegg reactome shinyapps

1 stars 5.36 score 22 scripts

bioc

MOSClip:Multi Omics Survival Clip

Topological pathway analysis tool able to integrate multi-omics data. It finds survival-associated modules or significant modules for two-class analysis. This tool have two main methods: pathway tests and module tests. The latter method allows the user to dig inside the pathways itself.

Maintained by Paolo Martini. Last updated 5 months ago.

software statisticalmethod graphandnetwork survival regression dimensionreduction pathways reactome

5.34 score 5 scripts

bioc

HybridExpress:Comparative analysis of RNA-seq data for hybrids and their progenitors

HybridExpress can be used to perform comparative transcriptomics analysis of hybrids (or allopolyploids) relative to their progenitor species. The package features functions to perform exploratory analyses of sample grouping, identify differentially expressed genes in hybrids relative to their progenitors, classify genes in expression categories (N = 12) and classes (N = 5), and perform functional analyses. We also provide users with graphical functions for the seamless creation of publication-ready figures that are commonly used in the literature.

Maintained by Fabricio Almeida-Silva. Last updated 5 months ago.

software functionalgenomics geneexpression transcriptomics rnaseq classification differentialexpression gene-expression hybrid polyploidy rna-seq

14 stars 5.32 score 2 scripts

bioc

hoodscanR:Spatial cellular neighbourhood scanning in R

hoodscanR is an user-friendly R package providing functions to assist cellular neighborhood analysis of any spatial transcriptomics data with single-cell resolution. All functions in the package are built based on the SpatialExperiment object, allowing integration into various spatial transcriptomics-related packages from Bioconductor. The package can result in cell-level neighborhood annotation output, along with funtions to perform neighborhood colocalization analysis and neighborhood-based cell clustering.

Maintained by Ning Liu. Last updated 2 months ago.

spatial transcriptomics singlecell clustering cpp

4 stars 5.32 score 15 scripts

bioc

bettr:A Better Way To Explore What Is Best

bettr provides a set of interactive visualization methods to explore the results of a benchmarking study, where typically more than a single performance measures are computed. The user can weight the performance measures according to their preferences. Performance measures can also be grouped and aggregated according to additional annotations.

Maintained by Charlotte Soneson. Last updated 5 months ago.

visualization shinyapps gui

3 stars 5.26 score 3 scripts

bioc

MultiRNAflow:An R package for integrated analysis of temporal RNA-seq data with multiple biological conditions

Our R package MultiRNAflow provides an easy to use unified framework allowing to automatically make both unsupervised and supervised (DE) analysis for datasets with an arbitrary number of biological conditions and time points. In particular, our code makes a deep downstream analysis of DE information, e.g. identifying temporal patterns across biological conditions and DE genes which are specific to a biological condition for each time.

Maintained by Rodolphe Loubaton. Last updated 5 months ago.

sequencing rnaseq geneexpression transcription timecourse preprocessing visualization normalization principalcomponent clustering differentialexpression genesetenrichment pathways

6 stars 5.26 score 4 scripts

bioc

CytoMDS:Low Dimensions projection of cytometry samples

This package implements a low dimensional visualization of a set of cytometry samples, in order to visually assess the 'distances' between them. This, in turn, can greatly help the user to identify quality issues like batch effects or outlier samples, and/or check the presence of potential sample clusters that might align with the exeprimental design. The CytoMDS algorithm combines, on the one hand, the concept of Earth Mover's Distance (EMD), a.k.a. Wasserstein metric and, on the other hand, the Multi Dimensional Scaling (MDS) algorithm for the low dimensional projection. Also, the package provides some diagnostic tools for both checking the quality of the MDS projection, as well as tools to help with the interpretation of the axes of the projection.

Maintained by Philippe Hauchamps. Last updated 2 months ago.

flowcytometry qualitycontrol dimensionreduction multidimensionalscaling software visualization

1 stars 5.23 score 2 scripts

bioc

BulkSignalR:Infer Ligand-Receptor Interactions from bulk expression (transcriptomics/proteomics) data, or spatial transcriptomics

Inference of ligand-receptor (LR) interactions from bulk expression (transcriptomics/proteomics) data, or spatial transcriptomics. BulkSignalR bases its inferences on the LRdb database included in our other package, SingleCellSignalR available from Bioconductor. It relies on a statistical model that is specific to bulk data sets. Different visualization and data summary functions are proposed to help navigating prediction results.

Maintained by Jean-Philippe Villemin. Last updated 3 months ago.

network rnaseq software proteomics transcriptomics networkinference spatial

5.22 score 15 scripts

frederikziebell

RNAseqQC:Quality Control for RNA-Seq Data

Functions for semi-automated quality control of bulk RNA-seq data.

Maintained by Frederik Ziebell. Last updated 9 months ago.

2 stars 5.21 score 27 scripts

bioc

Damsel:Damsel: an end to end analysis of DamID

Damsel provides an end to end analysis of DamID data. Damsel takes bam files from Dam-only control and fusion samples and counts the reads matching to each GATC region. edgeR is utilised to identify regions of enrichment in the fusion relative to the control. Enriched regions are combined into peaks, and are associated with nearby genes. Damsel allows for IGV style plots to be built as the results build, inspired by ggcoverage, and using the functionality and layering ability of ggplot2. Damsel also conducts gene ontology testing with bias correction through goseq, and future versions of Damsel will also incorporate motif enrichment analysis. Overall, Damsel is the first package allowing for an end to end analysis with visual capabilities. The goal of Damsel was to bring all the analysis into one place, and allow for exploratory analysis within R.

Maintained by Caitlin Page. Last updated 5 months ago.

differentialmethylation peakdetection geneprediction genesetenrichment

5.20 score 20 scripts

miriamesteve

GSSTDA:Progression Analysis of Disease with Survival using Topological Data Analysis

Mapper-based survival analysis with transcriptomics data is designed to carry out. Mapper-based survival analysis is a modification of Progression Analysis of Disease (PAD) where survival data is taken into account in the filtering function. More details in: J. Fores-Martos, B. Suay-Garcia, R. Bosch-Romeu, M.C. Sanfeliu-Alonso, A. Falco, J. Climent, "Progression Analysis of Disease with Survival (PAD-S) by SurvMap identifies different prognostic subgroups of breast cancer in a large combined set of transcriptomics and methylation studies" <doi:10.1101/2022.09.08.507080>.

Maintained by Miriam Esteve. Last updated 8 months ago.

2 stars 5.15 score 7 scripts

aalhendi1707

countToFPKM:Convert Counts to Fragments per Kilobase of Transcript per Million (FPKM)

Implements the algorithm described in Trapnell,C. et al. (2010) <doi: 10.1038/nbt.1621>. This function takes read counts matrix of RNA-Seq data, feature lengths which can be retrieved using 'biomaRt' package, and the mean fragment lengths which can be calculated using the 'CollectInsertSizeMetrics(Picard)' tool. It then returns a matrix of FPKM normalised data by library size and feature effective length. It also provides the user with a quick and reliable function to generate FPKM heatmap plot of the highly variable features in RNA-Seq dataset.

Maintained by Ahmed Alhendi. Last updated 4 years ago.

gene-expression normalization rna-seq

62 stars 5.09 score 20 scripts

bioc

chevreulShiny:Tools for managing SingleCellExperiment objects as projects

Tools for managing SingleCellExperiment objects as projects. Includes functions for analysis and visualization of single-cell data. Also included is a shiny app for visualization of pre-processed scRNA data. Supported by NIH grants R01CA137124 and R01EY026661 to David Cobrinik.

Maintained by Kevin Stachelek. Last updated 29 days ago.

coverage rnaseq sequencing visualization geneexpression transcription singlecell transcriptomics normalization preprocessing qualitycontrol dimensionreduction dataimport

5.08 score

bioc

CTexploreR:Explores Cancer Testis Genes

The CTexploreR package re-defines the list of Cancer Testis/Germline (CT) genes. It is based on publicly available RNAseq databases (GTEx, CCLE and TCGA) and summarises CT genes' main characteristics. Several visualisation functions allow to explore their expression in different types of tissues and cancer cells, or to inspect the methylation status of their promoters in normal tissues.

Maintained by Axelle Loriot. Last updated 5 months ago.

transcriptomics epigenetics differentialexpression geneexpression dnamethylation experimenthubsoftware dataimport bioconductor

5.02 score 2 scripts

bioc

recoup:An R package for the creation of complex genomic profile plots

recoup calculates and plots signal profiles created from short sequence reads derived from Next Generation Sequencing technologies. The profiles provided are either sumarized curve profiles or heatmap profiles. Currently, recoup supports genomic profile plots for reads derived from ChIP-Seq and RNA-Seq experiments. The package uses ggplot2 and ComplexHeatmap graphics facilities for curve and heatmap coverage profiles respectively.

Maintained by Panagiotis Moulos. Last updated 5 months ago.

immunooncology software geneexpression preprocessing qualitycontrol rnaseq chipseq sequencing coverage atacseq chiponchip alignment dataimport

1 stars 5.02 score 2 scripts

bioc

broadSeq:broadSeq : for streamlined exploration of RNA-seq data

This package helps user to do easily RNA-seq data analysis with multiple methods (usually which needs many different input formats). Here the user will provid the expression data as a SummarizedExperiment object and will get results from different methods. It will help user to quickly evaluate different methods.

Maintained by Rishi Das Roy. Last updated 5 months ago.

geneexpression differentialexpression rnaseq transcriptomics sequencing coverage genesetenrichment go

4 stars 5.00 score 7 scripts

bioc

InterCellar:InterCellar: an R-Shiny app for interactive analysis and exploration of cell-cell communication in single-cell transcriptomics

InterCellar is implemented as an R/Bioconductor Package containing a Shiny app that allows users to interactively analyze cell-cell communication from scRNA-seq data. Starting from precomputed ligand-receptor interactions, InterCellar provides filtering options, annotations and multiple visualizations to explore clusters, genes and functions. Finally, based on functional annotation from Gene Ontology and pathway databases, InterCellar implements data-driven analyses to investigate cell-cell communication in one or multiple conditions.

Maintained by Marta Interlandi. Last updated 5 months ago.

software singlecell visualization go transcriptomics

9 stars 4.95 score 7 scripts

bioc

iSEEpathways:iSEE extension for panels related to pathway analysis

This package contains diverse functionality to extend the usage of the iSEE package, including additional classes for the panels or modes facilitating the analysis of pathway analysis results. This package does not perform pathway analysis. Instead, it provides methods to embed precomputed pathway analysis results in a SummarizedExperiment object, in a manner that is compatible with interactive visualisation in iSEE applications.

Maintained by Kevin Rue-Albrecht. Last updated 5 months ago.

software infrastructure differentialexpression geneexpression gui visualization pathways genesetenrichment go shinyapps bioconductor hacktoberfest isee iseeu

1 stars 4.95 score 10 scripts

bioc

epiregulon.extra:Companion package to epiregulon with additional plotting, differential and graph functions

Gene regulatory networks model the underlying gene regulation hierarchies that drive gene expression and observed phenotypes. Epiregulon infers TF activity in single cells by constructing a gene regulatory network (regulons). This is achieved through integration of scATAC-seq and scRNA-seq data and incorporation of public bulk TF ChIP-seq data. Links between regulatory elements and their target genes are established by computing correlations between chromatin accessibility and gene expressions.

Maintained by Xiaosai Yao. Last updated 13 days ago.

generegulation network geneexpression transcription chiponchip differentialexpression genetarget normalization graphandnetwork

4.95 score 10 scripts

bioc

decontX:Decontamination of single cell genomics data

This package contains implementation of DecontX (Yang et al. 2020), a decontamination algorithm for single-cell RNA-seq, and DecontPro (Yin et al. 2023), a decontamination algorithm for single cell protein expression data. DecontX is a novel Bayesian method to computationally estimate and remove RNA contamination in individual cells without empty droplet information. DecontPro is a Bayesian method that estimates the level of contamination from ambient and background sources in CITE-seq ADT dataset and decontaminate the dataset.

Maintained by Joshua Campbell. Last updated 1 months ago.

singlecell bayesian cpp

4.94 score 29 scripts

bioc

MetaboSignal:MetaboSignal: a network-based approach to overlay and explore metabolic and signaling KEGG pathways

MetaboSignal is an R package that allows merging, analyzing and customizing metabolic and signaling KEGG pathways. It is a network-based approach designed to explore the topological relationship between genes (signaling- or enzymatic-genes) and metabolites, representing a powerful tool to investigate the genetic landscape and regulatory networks of metabolic phenotypes.

Maintained by Andrea Rodriguez-Martinez. Last updated 5 months ago.

graphandnetwork genesignaling genetarget network pathways kegg reactome software

4.90 score 8 scripts

andrewdhawan

sigQC:Quality Control Metrics for Gene Signatures

Provides gene signature quality control metrics in publication ready plots. Namely, enables the visualization of properties such as expression, variability, correlation, and comparison of methods of standardisation and scoring metrics.

Maintained by Andrew Dhawan. Last updated 8 months ago.

4 stars 4.89 score 13 scripts

bioc

AMARETTO:Regulatory Network Inference and Driver Gene Evaluation using Integrative Multi-Omics Analysis and Penalized Regression

Integrating an increasing number of available multi-omics cancer data remains one of the main challenges to improve our understanding of cancer. One of the main challenges is using multi-omics data for identifying novel cancer driver genes. We have developed an algorithm, called AMARETTO, that integrates copy number, DNA methylation and gene expression data to identify a set of driver genes by analyzing cancer samples and connects them to clusters of co-expressed genes, which we define as modules. We applied AMARETTO in a pancancer setting to identify cancer driver genes and their modules on multiple cancer sites. AMARETTO captures modules enriched in angiogenesis, cell cycle and EMT, and modules that accurately predict survival and molecular subtypes. This allows AMARETTO to identify novel cancer driver genes directing canonical cancer pathways.

Maintained by Olivier Gevaert. Last updated 5 months ago.

statisticalmethod differentialmethylation generegulation geneexpression methylationarray transcription preprocessing batcheffect dataimport mrnamicroarray micrornaarray regression clustering rnaseq copynumbervariation sequencing microarray normalization network bayesian exonarray onechannel twochannel proprietaryplatforms alternativesplicing differentialexpression differentialsplicing genesetenrichment multiplecomparison qualitycontrol timecourse

4.88 score 15 scripts

rhenkin

visxhclust:A Shiny App for Visual Exploration of Hierarchical Clustering

A Shiny application and functions for visual exploration of hierarchical clustering with numeric datasets. Allows users to iterative set hyperparameters, select features and evaluate results through various plots and computation of evaluation criteria.

Maintained by Rafael Henkin. Last updated 2 years ago.

clustering data-analysis data-science r-shiny shiny-apps

4 stars 4.86 score 12 scripts

bioc

multistateQTL:Toolkit for the analysis of multi-state QTL data

A collection of tools for doing various analyses of multi-state QTL data, with a focus on visualization and interpretation. The package 'multistateQTL' contains functions which can remove or impute missing data, identify significant associations, as well as categorise features into global, multi-state or unique. The analysis results are stored in a 'QTLExperiment' object, which is based on the 'SummarisedExperiment' framework.

Maintained by Amelia Dunstone. Last updated 13 days ago.

functionalgenomics geneexpression sequencing visualization snp software

1 stars 4.81 score 9 scripts

moseleybioinformaticslab

visualizationQualityControl:Development of visualization methods for quality control

Provides utilities useful quality control of high-throughput -omics datasets.

Maintained by Robert M Flight. Last updated 1 years ago.

bioinformatics correlation quality-control visualization

10 stars 4.78 score 30 scripts

bioc

gINTomics:Multi-Omics data integration

gINTomics is an R package for Multi-Omics data integration and visualization. gINTomics is designed to detect the association between the expression of a target and of its regulators, taking into account also their genomics modifications such as Copy Number Variations (CNV) and methylation. What is more, gINTomics allows integration results visualization via a Shiny-based interactive app.

Maintained by Angelo Velle. Last updated 5 months ago.

geneexpression rnaseq microarray visualization copynumbervariation genetarget quarto

3 stars 4.78 score 3 scripts

bioc

airpart:Differential cell-type-specific allelic imbalance

Airpart identifies sets of genes displaying differential cell-type-specific allelic imbalance across cell types or states, utilizing single-cell allelic counts. It makes use of a generalized fused lasso with binomial observations of allelic counts to partition cell types by their allelic imbalance. Alternatively, a nonparametric method for partitioning cell types is offered. The package includes a number of visualizations and quality control functions for examining single cell allelic imbalance datasets.

Maintained by Wancen Mu. Last updated 5 months ago.

singlecell rnaseq atacseq chipseq sequencing generegulation geneexpression transcription transcriptomevariant cellbiology functionalgenomics differentialexpression graphandnetwork regression clustering qualitycontrol

2 stars 4.78 score 2 scripts

bioc

MatrixQCvis:Shiny-based interactive data-quality exploration for omics data

Data quality assessment is an integral part of preparatory data analysis to ensure sound biological information retrieval. We present here the MatrixQCvis package, which provides shiny-based interactive visualization of data quality metrics at the per-sample and per-feature level. It is broadly applicable to quantitative omics data types that come in matrix-like format (features x samples). It enables the detection of low-quality samples, drifts, outliers and batch effects in data sets. Visualizations include amongst others bar- and violin plots of the (count/intensity) values, mean vs standard deviation plots, MA plots, empirical cumulative distribution function (ECDF) plots, visualizations of the distances between samples, and multiple types of dimension reduction plots. Furthermore, MatrixQCvis allows for differential expression analysis based on the limma (moderated t-tests) and proDA (Wald tests) packages. MatrixQCvis builds upon the popular Bioconductor SummarizedExperiment S4 class and enables thus the facile integration into existing workflows. The package is especially tailored towards metabolomics and proteomics mass spectrometry data, but also allows to assess the data quality of other data types that can be represented in a SummarizedExperiment object.

Maintained by Thomas Naake. Last updated 5 months ago.

visualization shinyapps gui qualitycontrol dimensionreduction metabolomics proteomics transcriptomics

4.74 score 4 scripts

bioc

MesKit:A tool kit for dissecting cancer evolution from multi-region derived tumor biopsies via somatic alterations

MesKit provides commonly used analysis and visualization modules based on mutational data generated by multi-region sequencing (MRS). This package allows to depict mutational profiles, measure heterogeneity within or between tumors from the same patient, track evolutionary dynamics, as well as characterize mutational patterns on different levels. Shiny application was also developed for a need of GUI-based analysis. As a handy tool, MesKit can facilitate the interpretation of tumor heterogeneity and the understanding of evolutionary relationship between regions in MRS study.

Maintained by Mengni Liu. Last updated 5 months ago.

4.73 score 18 scripts 1 dependents

bioc

CytoPipelineGUI:GUI's for visualization of flow cytometry data analysis pipelines

This package is the companion of the `CytoPipeline` package. It provides GUI's (shiny apps) for the visualization of flow cytometry data analysis pipelines that are run with `CytoPipeline`. Two shiny applications are provided, i.e. an interactive flow frame assessment and comparison tool and an interactive scale transformations visualization and adjustment tool.

Maintained by Philippe Hauchamps. Last updated 5 months ago.

flowcytometry preprocessing qualitycontrol workflowstep immunooncology software visualization gui shinyapps

4.70 score 2 scripts

bioc

ELViS:An R Package for Estimating Copy Number Levels of Viral Genome Segments Using Base-Resolution Read Depth Profile

Base-resolution copy number analysis of viral genome. Utilizes base-resolution read depth data over viral genome to find copy number segments with two-dimensional segmentation approach. Provides publish-ready figures, including histograms of read depths, coverage line plots over viral genome annotated with copy number change events and viral genes, and heatmaps showing multiple types of data with integrative clustering of samples.

Maintained by Jin-Young Lee. Last updated 28 days ago.

copynumbervariation coverage genomicvariation biomedicalinformatics sequencing normalization visualization clustering

4.70 score 7 scripts

bioc

geyser:Gene Expression displaYer of SummarizedExperiment in R

Lightweight Expression displaYer (plotter / viewer) of SummarizedExperiment object in R. This package provides a quick and easy Shiny-based GUI to empower a user to use a SummarizedExperiment object to view (gene) expression grouped from the sample metadata columns (in the `colData` slot). Feature expression can either be viewed with a box plot or a heatmap.

Maintained by David McGaughey. Last updated 3 months ago.

software shinyapps gui geneexpression

4.65 score 18 scripts

bioc

iSEEfier:Streamlining the creation of initial states for starting an iSEE instance

iSEEfier provides a set of functionality to quickly and intuitively create, inspect, and combine initial configuration objects. These can be conveniently passed in a straightforward manner to the function call to launch iSEE() with the specified configuration. This package currently works seamlessly with the sets of panels provided by the iSEE and iSEEu packages, but can be extended to accommodate the usage of any custom panel (e.g. from iSEEde, iSEEpathways, or any panel developed independently by the user).

Maintained by Najla Abassi. Last updated 5 months ago.

cellbasedassays clustering dimensionreduction featureextraction gui geneexpression immunooncology shinyapps singlecell software transcription transcriptomics visualization

4.60 score 2 scripts

stamats

MKomics:Omics Data Analysis

Similarity plots based on correlation and median absolute deviation (MAD); adjusting colors for heatmaps; aggregate technical replicates; calculate pairwise fold-changes and log fold-changes; compute one- and two-way ANOVA; simplified interface to package 'limma' (Ritchie et al. (2015), <doi:10.1093/nar/gkv007> ) for moderated t-test and one-way ANOVA; Hamming and Levenshtein (edit) distance of strings as well as optimal alignment scores for global (Needleman-Wunsch) and local (Smith-Waterman) alignments with constant gap penalties (Merkl and Waack (2009), ISBN:978-3-527-32594-8).

Maintained by Matthias Kohl. Last updated 2 years ago.

3 stars 4.56 score 24 scripts

bioc

treekoR:Cytometry Cluster Hierarchy and Cellular-to-phenotype Associations

treekoR is a novel framework that aims to utilise the hierarchical nature of single cell cytometry data to find robust and interpretable associations between cell subsets and patient clinical end points. These associations are aimed to recapitulate the nested proportions prevalent in workflows inovlving manual gating, which are often overlooked in workflows using automatic clustering to identify cell populations. We developed treekoR to: Derive a hierarchical tree structure of cell clusters; quantify a cell types as a proportion relative to all cells in a sample (%total), and, as the proportion relative to a parent population (%parent); perform significance testing using the calculated proportions; and provide an interactive html visualisation to help highlight key results.

Maintained by Adam Chan. Last updated 5 months ago.

clustering differentialexpression flowcytometry immunooncology massspectrometry singlecell software statisticalmethod visualization

4.56 score 12 scripts 1 dependents

bioc

MAPFX:MAssively Parallel Flow cytometry Xplorer (MAPFX): A Toolbox for Analysing Data from the Massively-Parallel Cytometry Experiments

MAPFX is an end-to-end toolbox that pre-processes the raw data from MPC experiments (e.g., BioLegend's LEGENDScreen and BD Lyoplates assays), and further imputes the ‘missing’ infinity markers in the wells without those measurements. The pipeline starts by performing background correction on raw intensities to remove the noise from electronic baseline restoration and fluorescence compensation by adapting a normal-exponential convolution model. Unwanted technical variation, from sources such as well effects, is then removed using a log-normal model with plate, column, and row factors, after which infinity markers are imputed using the informative backbone markers as predictors. The completed dataset can then be used for clustering and other statistical analyses. Additionally, MAPFX can be used to normalise data from FFC assays as well.

Maintained by Hsiao-Chi Liao. Last updated 5 months ago.

software flowcytometry cellbasedassays singlecell proteomics clustering

1 stars 4.54 score

core-bioinformatics

bulkAnalyseR:Interactive Shiny App for Bulk Sequencing Data

Given an expression matrix from a bulk sequencing experiment, pre-processes it and creates a shiny app for interactive data analysis and visualisation. The app contains quality checks, differential expression analysis, volcano and cross plots, enrichment analysis and gene regulatory network inference, and can be customised to contain more panels by the user.

Maintained by Ilias Moutsopoulos. Last updated 1 years ago.

27 stars 4.47 score 11 scripts

gabrielelubatti

MitoHEAR:Quantification of Mitochondrial DNA Heteroplasmy

R package that allows the estimation and downstream statistical analysis of the mitochondrial DNA Heteroplasmy calculated from single-cell datasets.

Maintained by Gabriele Lubatti. Last updated 3 years ago.

software

4.45 score 14 scripts

bioc

PRONE:The PROteomics Normalization Evaluator

High-throughput omics data are often affected by systematic biases introduced throughout all the steps of a clinical study, from sample collection to quantification. Normalization methods aim to adjust for these biases to make the actual biological signal more prominent. However, selecting an appropriate normalization method is challenging due to the wide range of available approaches. Therefore, a comparative evaluation of unnormalized and normalized data is essential in identifying an appropriate normalization strategy for a specific data set. This R package provides different functions for preprocessing, normalizing, and evaluating different normalization approaches. Furthermore, normalization methods can be evaluated on downstream steps, such as differential expression analysis and statistical enrichment analysis. Spike-in data sets with known ground truth and real-world data sets of biological experiments acquired by either tandem mass tag (TMT) or label-free quantification (LFQ) can be analyzed.

Maintained by Lis Arend. Last updated 11 days ago.

proteomics preprocessing normalization differentialexpression visualization data-analysis evaluation

2 stars 4.41 score 9 scripts

niaid

HDStIM:High Dimensional Stimulation Immune Mapping ('HDStIM')

A method for identifying responses to experimental stimulation in mass or flow cytometry that uses high dimensional analysis of measured parameters and can be performed with an end-to-end unsupervised approach. In the context of in vitro stimulation assays where high-parameter cytometry was used to monitor intracellular response markers, using cell populations annotated either through automated clustering or manual gating for a combined set of stimulated and unstimulated samples, 'HDStIM' labels cells as responding or non-responding. The package also provides auxiliary functions to rank intracellular markers based on their contribution to identifying responses and generating diagnostic plots.

Maintained by Rohit Farmer. Last updated 1 years ago.

complexheatmap assay cytof cytometry cytometry-analysis-pipeline flowcytometry stimulation

3 stars 4.41 score 17 scripts

bioc

ASURAT:Functional annotation-driven unsupervised clustering for single-cell data

ASURAT is a software for single-cell data analysis. Using ASURAT, one can simultaneously perform unsupervised clustering and biological interpretation in terms of cell type, disease, biological process, and signaling pathway activity. Inputting a single-cell RNA-seq data and knowledge-based databases, such as Cell Ontology, Gene Ontology, KEGG, etc., ASURAT transforms gene expression tables into original multivariate tables, termed sign-by-sample matrices (SSMs).

Maintained by Keita Iida. Last updated 5 months ago.

geneexpression singlecell sequencing clustering genesignaling cpp

4.32 score 21 scripts

loosolab

wilson:Web-Based Interactive Omics Visualization

Tool-set of modules for creating web-based applications that use plot based strategies to visualize and analyze multi-omics data. This package utilizes the 'shiny' and 'plotly' frameworks to provide a user friendly dashboard for interactive plotting.

Maintained by Hendrik Schultheis. Last updated 4 years ago.

2 stars 4.30 score 7 scripts

bioc

cytofQC:Labels normalized cells for CyTOF data and assigns probabilities for each label

cytofQC is a package for initial cleaning of CyTOF data. It uses a semi-supervised approach for labeling cells with their most likely data type (bead, doublet, debris, dead) and the probability that they belong to each label type. This package does not remove data from the dataset, but provides labels and information to aid the data user in cleaning their data. Our algorithm is able to distinguish between doublets and large cells.

Maintained by Jill Lundell. Last updated 5 months ago.

software singlecell annotation

2 stars 4.30 score 3 scripts

bioc

censcyt:Differential abundance analysis with a right censored covariate in high-dimensional cytometry

Methods for differential abundance analysis in high-dimensional cytometry data when a covariate is subject to right censoring (e.g. survival time) based on multiple imputation and generalized linear mixed models.

Maintained by Reto Gerber. Last updated 5 months ago.

immunooncology flowcytometry proteomics singlecell cellbasedassays cellbiology clustering featureextraction software survival

4.30 score 2 scripts

bioc

gmoviz:Seamless visualization of complex genomic variations in GMOs and edited cell lines

Genetically modified organisms (GMOs) and cell lines are widely used models in all kinds of biological research. As part of characterising these models, DNA sequencing technology and bioinformatics analyses are used systematically to study their genomes. Therefore, large volumes of data are generated and various algorithms are applied to analyse this data, which introduces a challenge on representing all findings in an informative and concise manner. `gmoviz` provides users with an easy way to visualise and facilitate the explanation of complex genomic editing events on a larger, biologically-relevant scale.

Maintained by Kathleen Zeglinski. Last updated 5 months ago.

visualization sequencing geneticvariability genomicvariation coverage

4.30 score 9 scripts

bioc

BloodGen3Module:This R package for performing module repertoire analyses and generating fingerprint representations

The BloodGen3Module package provides functions for R user performing module repertoire analyses and generating fingerprint representations. Functions can perform group comparison or individual sample analysis and visualization by fingerprint grid plot or fingerprint heatmap. Module repertoire analyses typically involve determining the percentage of the constitutive genes for each module that are significantly increased or decreased. As we describe in details;https://www.biorxiv.org/content/10.1101/525709v2 and https://pubmed.ncbi.nlm.nih.gov/33624743/, the results of module repertoire analyses can be represented in a fingerprint format, where red and blue spots indicate increases or decreases in module activity. These spots are subsequently represented either on a grid, with each position being assigned to a given module, or in a heatmap where the samples are arranged in columns and the modules in rows.

Maintained by Darawan Rinchai. Last updated 5 months ago.

software visualization geneexpression

4.30 score 5 scripts

bioc

spillR:Spillover Compensation in Mass Cytometry Data

Channel interference in mass cytometry can cause spillover and may result in miscounting of protein markers. We develop a nonparametric finite mixture model and use the mixture components to estimate the probability of spillover. We implement our method using expectation-maximization to fit the mixture model.

Maintained by Marco Guazzini. Last updated 5 months ago.

flowcytometry immunooncology massspectrometry preprocessing singlecell software statisticalmethod visualization regression

4.30 score 3 scripts

bioc

cageminer:Candidate Gene Miner

This package aims to integrate GWAS-derived SNPs and coexpression networks to mine candidate genes associated with a particular phenotype. For that, users must define a set of guide genes, which are known genes involved in the studied phenotype. Additionally, the mined candidates can be given a score that favor candidates that are hubs and/or transcription factors. The scores can then be used to rank and select the top n most promising genes for downstream experiments.

Maintained by Fabrício Almeida-Silva. Last updated 5 months ago.

software snp functionalprediction genomewideassociation geneexpression networkenrichment variantannotation functionalgenomics network

1 stars 4.30 score 5 scripts

bioc

blacksheepr:Outlier Analysis for pairwise differential comparison

Blacksheep is a tool designed for outlier analysis in the context of pairwise comparisons in an effort to find distinguishing characteristics from two groups. This tool was designed to be applied for biological applications such as phosphoproteomics or transcriptomics, but it can be used for any data that can be represented by a 2D table, and has two sub populations within the table to compare.

Maintained by RugglesLab. Last updated 5 months ago.

sequencing rnaseq geneexpression transcription differentialexpression transcriptomics

4.30 score 6 scripts

bioc

CeTF:Coexpression for Transcription Factors using Regulatory Impact Factors and Partial Correlation and Information Theory analysis

This package provides the necessary functions for performing the Partial Correlation coefficient with Information Theory (PCIT) (Reverter and Chan 2008) and Regulatory Impact Factors (RIF) (Reverter et al. 2010) algorithm. The PCIT algorithm identifies meaningful correlations to define edges in a weighted network and can be applied to any correlation-based network including but not limited to gene co-expression networks, while the RIF algorithm identify critical Transcription Factors (TF) from gene expression data. These two algorithms when combined provide a very relevant layer of information for gene expression studies (Microarray, RNA-seq and single-cell RNA-seq data).

Maintained by Carlos Alberto Oliveira de Biagi Junior. Last updated 5 months ago.

sequencing rnaseq microarray geneexpression transcription normalization differentialexpression singlecell network regression chipseq immunooncology coverage cpp

4.30 score 9 scripts

bioc

CyTOFpower:Power analysis for CyTOF experiments

This package is a tool to predict the power of CyTOF experiments in the context of differential state analyses. The package provides a shiny app with two options to predict the power of an experiment: i. generation of in-sicilico CyTOF data, using users input ii. browsing in a grid of parameters for which the power was already precomputed.

Maintained by Anne-Maud Ferreira. Last updated 17 days ago.

flowcytometry singlecell cellbiology statisticalmethod software

4.18 score 2 scripts

bioc

MPAC:Multi-omic Pathway Analysis of Cells

Multi-omic Pathway Analysis of Cells (MPAC), integrates multi-omic data for understanding cellular mechanisms. It predicts novel patient groups with distinct pathway profiles as well as identifying key pathway proteins with potential clinical associations. From CNA and RNA-seq data, it determines genes’ DNA and RNA states (i.e., repressed, normal, or activated), which serve as the input for PARADIGM to calculate Inferred Pathway Levels (IPLs). It also permutes DNA and RNA states to create a background distribution to filter IPLs as a way to remove events observed by chance. It provides multiple methods for downstream analysis and visualization.

Maintained by Peng Liu. Last updated 18 days ago.

software technology sequencing rnaseq survival clustering immunooncology

4.18 score 1 scripts

bioc

dinoR:Differential NOMe-seq analysis

dinoR tests for significant differences in NOMe-seq footprints between two conditions, using genomic regions of interest (ROI) centered around a landmark, for example a transcription factor (TF) motif. This package takes NOMe-seq data (GCH methylation/protection) in the form of a Ranged Summarized Experiment as input. dinoR can be used to group sequencing fragments into 3 or 5 categories representing characteristic footprints (TF bound, nculeosome bound, open chromatin), plot the percentage of fragments in each category in a heatmap, or averaged across different ROI groups, for example, containing a common TF motif. It is designed to compare footprints between two sample groups, using edgeR's quasi-likelihood methods on the total fragment counts per ROI, sample, and footprint category.

Maintained by Michaela Schwaiger. Last updated 5 months ago.

nucleosomepositioning epigenetics methylseq differentialmethylation coverage transcription sequencing software

4.18 score 7 scripts

airr-community

ogrdbstats:Analysis of Adaptive Immune Receptor Repertoire Germ Line Statistics

Multiple tools are now available for inferring the personalised germ line set from an adaptive immune receptor repertoire. Output from these tools is converted to a single format and supplemented with rich data such as usage and characterisation of 'novel' germ line alleles. This data can be particularly useful when considering the validity of novel inferences. Use of the analysis provided is described in <doi:10.3389/fimmu.2019.00435>.

Maintained by William Lees. Last updated 1 months ago.

1 stars 4.18 score 8 scripts

bioc

profileplyr:Visualization and annotation of read signal over genomic ranges with profileplyr

Quick and straightforward visualization of read signal over genomic intervals is key for generating hypotheses from sequencing data sets (e.g. ChIP-seq, ATAC-seq, bisulfite/methyl-seq). Many tools both inside and outside of R and Bioconductor are available to explore these types of data, and they typically start with a bigWig or BAM file and end with some representation of the signal (e.g. heatmap). profileplyr leverages many Bioconductor tools to allow for both flexibility and additional functionality in workflows that end with visualization of the read signal.

Maintained by Tom Carroll. Last updated 5 months ago.

chipseq dataimport sequencing chiponchip coverage

4.03 score 54 scripts

bioc

cytoKernel:Differential expression using kernel-based score test

cytoKernel implements a kernel-based score test to identify differentially expressed features in high-dimensional biological experiments. This approach can be applied across many different high-dimensional biological data including gene expression data and dimensionally reduced cytometry-based marker expression data. In this R package, we implement functions that compute the feature-wise p values and their corresponding adjusted p values. Additionally, it also computes the feature-wise shrunk effect sizes and their corresponding shrunken effect size. Further, it calculates the percent of differentially expressed features and plots user-friendly heatmap of the top differentially expressed features on the rows and samples on the columns.

Maintained by Tusharkanti Ghosh. Last updated 5 months ago.

immunooncology proteomics singlecell software onechannel flowcytometry differentialexpression geneexpression clustering cpp

4.00 score 4 scripts

bioc

fCCAC:functional Canonical Correlation Analysis to evaluate Covariance between nucleic acid sequencing datasets

Computational evaluation of variability across DNA or RNA sequencing datasets is a crucial step in genomics, as it allows both to evaluate reproducibility of replicates, and to compare different datasets to identify potential correlations. fCCAC applies functional Canonical Correlation Analysis to allow the assessment of: (i) reproducibility of biological or technical replicates, analyzing their shared covariance in higher order components; and (ii) the associations between different datasets. fCCAC represents a more sophisticated approach that complements Pearson correlation of genomic coverage.

Maintained by Pedro Madrigal. Last updated 5 months ago.

epigenetics transcription sequencing coverage chipseq functionalgenomics rnaseq atacseq mnaseseq

4.00 score 1 scripts

mpallocc

autoGO:Auto-GO: Reproducible, Robust and High Quality Ontology Enrichment Visualizations

Auto-GO is a framework that enables automated, high quality Gene Ontology enrichment analysis visualizations. It also features a handy wrapper for Differential Expression analysis around the 'DESeq2' package described in Love et al. (2014) <doi:10.1186/s13059-014-0550-8>. The whole framework is structured in different, independent functions, in order to let the user decide which steps of the analysis to perform and which plot to produce.

Maintained by Fabio Ticconi. Last updated 1 months ago.

2 stars 3.90 score

ecortesgomez

DiscreteGapStatistic:An Extension of the Gap Statistic for Ordinal/Categorical Data

The gap statistic approach is extended to estimate the number of clusters for categorical response format data. This approach and accompanying software is designed to be used with the output of any clustering algorithm and with distances specifically designed for categorical (i.e. multiple choice) or ordinal survey response data.

Maintained by Eduardo Cortes. Last updated 27 days ago.

3.81 score 4 scripts

bioc

MWASTools:MWASTools: an integrated pipeline to perform metabolome-wide association studies

MWASTools provides a complete pipeline to perform metabolome-wide association studies. Key functionalities of the package include: quality control analysis of metabonomic data; MWAS using different association models (partial correlations; generalized linear models); model validation using non-parametric bootstrapping; visualization of MWAS results; NMR metabolite identification using STOCSY; and biological interpretation of MWAS results.

Maintained by Andrea Rodriguez-Martinez. Last updated 5 months ago.

metabolomics lipidomics cheminformatics systemsbiology qualitycontrol

3.78 score 5 scripts 1 dependents

bioc

ClustAll:ClustAll: Data driven strategy to robustly identify stratification of patients within complex diseases

Data driven strategy to find hidden groups of patients with complex diseases using clinical data. ClustAll facilitates the unsupervised identification of multiple robust stratifications. ClustAll, is able to overcome the most common limitations found when dealing with clinical data (missing values, correlated data, mixed data types).

Maintained by Asier Ortega-Legarreta. Last updated 5 months ago.

software statisticalmethod clustering dimensionreduction principalcomponent

3.70 score 1 scripts

yixiao-zeng

missoNet:Missingness in Multi-Task Regression with Network Estimation

Efficient procedures for fitting conditional graphical lasso models that link a set of predictor variables to a set of response variables (or tasks), even when the response data may contain missing values. 'missoNet' simultaneously estimates the predictor coefficients for all tasks by leveraging information from one another, in order to provide more accurate predictions in comparison to modeling them individually. Additionally, 'missoNet' estimates the response network structure influenced by conditioning predictor variables using a L1-regularized conditional Gaussian graphical model. Unlike most penalized multi-task regression methods (e.g., MRCE), 'missoNet' is capable of obtaining estimates even when the response data is corrupted by missing values. The method automatically enjoys the theoretical and computational benefits of convexity, and returns solutions that are comparable to the estimates obtained without missingness.

Maintained by Yixiao Zeng. Last updated 2 years ago.

conditional-graphical-lasso missing-data multi-task-regression openblas cpp

1 stars 3.70 score 2 scripts

bioc

segmenter:Perform Chromatin Segmentation Analysis in R by Calling ChromHMM

Chromatin segmentation analysis transforms ChIP-seq data into signals over the genome. The latter represents the observed states in a multivariate Markov model to predict the chromatin's underlying states. ChromHMM, written in Java, integrates histone modification datasets to learn the chromatin states de-novo. The goal of this package is to call chromHMM from within R, capture the output files in an S4 object and interface to other relevant Bioconductor analysis tools. In addition, segmenter provides functions to test, select and visualize the output of the segmentation.

Maintained by Mahmoud Ahmed. Last updated 5 months ago.

software histonemodification bioconductor chromhmm segmentation-an

4 stars 3.60 score 9 scripts

cogdisreslab

PAVER:PAVER: Pathway Analysis Visualization with Embedding Representations

Summary visualization using embedding representations to reveal underlying themes within sets of pathway terms.

Maintained by William G Ryan V. Last updated 8 months ago.

3.48 score 6 scripts

bioc

gCrisprTools:Suite of Functions for Pooled Crispr Screen QC and Analysis

Set of tools for evaluating pooled high-throughput screening experiments, typically employing CRISPR/Cas9 or shRNA expression cassettes. Contains methods for interrogating library and cassette behavior within an experiment, identifying differentially abundant cassettes, aggregating signals to identify candidate targets for empirical validation, hypothesis testing, and comprehensive reporting. Version 2.0 extends these applications to include a variety of tools for contextualizing and integrating signals across many experiments, incorporates extended signal enrichment methodologies via the "sparrow" package, and streamlines many formal requirements to aid in interpretablity.

Maintained by Russell Bainer. Last updated 5 months ago.

immunooncology crispr pooledscreens experimentaldesign biomedicalinformatics cellbiology functionalgenomics pharmacogenomics pharmacogenetics systemsbiology differentialexpression genesetenrichment genetics multiplecomparison normalization preprocessing qualitycontrol rnaseq regression software visualization

3.30 score 8 scripts

abdel-elsayed87

GRIN2:Genomic Random Interval (GRIN)

Improved version of 'GRIN' software that streamlines its use in practice to analyze genomic lesion data, accelerate its computing, and expand its analysis capabilities to answer additional scientific questions including a rigorous evaluation of the association of genomic lesions with RNA expression. Pounds, Stan, et al. (2013) <DOI:10.1093/bioinformatics/btt372>.

Maintained by Abdelrahman Elsayed. Last updated 4 months ago.

3.30 score

kumes

GoogleImage2Array:Create Array Data from 2D Image Thumbnails via Google Image Search

Images are provided as an array dataset of 2D image thumbnails from Google Image Search <https://www.google.com/search>. This array data may be suitable for a training data of machine learning or deep learning as a first trial.

Maintained by Satoshi Kume. Last updated 2 years ago.

image

3 stars 3.18 score 1 scripts

joeymays

karyotapR:DNA Copy Number Analysis for Genome-Wide Tapestri Panels

Analysis of DNA copy number in single cells using custom genome-wide targeted DNA sequencing panels for the Mission Bio Tapestri platform. Users can easily parse, manipulate, and visualize datasets produced from the automated 'Tapestri Pipeline', with support for normalization, clustering, and copy number calling. Functions are also available to deconvolute multiplexed samples by genotype and parsing barcoded reads from exogenous lentiviral constructs.

Maintained by Joseph Mays. Last updated 1 years ago.

1 stars 3.16 score 29 scripts

geertsmanon

rKOMICS:Minicircle Sequence Cluster (MSC) Analyses

It establishes a critical framework to manipulate, explore and extract biologically relevant information from mitochondrial minicircle assemblies in tens to hundreds of samples simultaneously and efficiently. This should facilitate research that aims to develop new molecular markers for identifying species-specific minicircles, or to study the ancestry of parasites for complementary insights into their evolutionary history.

Maintained by Manon Geerts. Last updated 4 years ago.

3.00 score 2 scripts

zhuxr11

mineSweepR:Mine Sweeper Game

This is the very popular mine sweeper game! The game requires you to find out tiles that contain mines through clues from unmasking neighboring tiles. Each tile that does not contain a mine shows the number of mines in its adjacent tiles. If you unmask all tiles that do not contain mines, you win the game; if you unmask any tile that contains a mine, you lose the game. For further game instructions, please run `help(run_game)` and check details. This game runs in X11-compatible devices with `grDevices::x11()`.

Maintained by Xiurui Zhu. Last updated 1 years ago.

2.70 score 1 scripts

abeyrann

Path.Analysis:Path Coefficient Analysis

Facilitates the performance of several analyses, including simple and sequential path coefficient analysis, correlation estimate, drawing correlogram, Heatmap, and path diagram. When working with raw data, that includes one or more dependent variables along with one or more independent variables are available, the path coefficient analysis can be conducted. It allows for testing direct effects, which can be a vital indicator in path coefficient analysis. The process of preparing the dataset rule is explained in detail in the vignette file "Path.Analysis_manual.Rmd". You can find this in the folders labelled "data" and "~/inst/extdata". Also see: 1)the 'lavaan', 2)a sample of sequential path analysis in 'metan' suggested by Olivoto and Lúcio (2020) <doi:10.1111/2041-210X.13384>, 3)the simple 'PATHSAS' macro written in 'SAS' by Cramer et al. (1999) <doi:10.1093/jhered/90.1.260>, and 4)the semPlot() function of 'OpenMx' as initial tools for conducting path coefficient analyses and SEM (Structural Equation Modeling). To gain a comprehensive understanding of path coefficient analysis, both in theory and practice, see a 'Minitab' macro developed by Arminian, A. in the paper by Arminian et al. (2008) <doi:10.1080/15427520802043182>.

Maintained by Ali Arminian. Last updated 6 months ago.

2.70 score

csoneson

ConfoundingExplorer:Confounding Explorer

This package provides a simple interactive application for investigating the effect of confounding between a signal of interest and a batch effect. It uses simulated data with user-specified effect sizes for both batch and condition effects. The user can also specify the number of samples in each condition and batch, and thereby the degree of confounding.

Maintained by Charlotte Soneson. Last updated 3 months ago.

regression experimentaldesign multiplecomparison batcheffect

2 stars 2.60 score 3 scripts

rajkumpismb

PCAPAM50:Enhanced 'PAM50' Subtyping of Breast Cancer

Accurate classification of breast cancer tumors based on gene expression data is not a trivial task, and it lacks standard practices.The 'PAM50' classifier, which uses 50 gene centroid correlation distances to classify tumors, faces challenges with balancing estrogen receptor (ER) status and gene centering. The 'PCAPAM50' package leverages principal component analysis and iterative 'PAM50' calls to create a gene expression-based ER-balanced subset for gene centering, avoiding the use of protein expression-based ER data resulting into an enhanced Breast Cancer subtyping.

Maintained by Praveen-Kumar Raj-Kumar. Last updated 3 months ago.

2.48 score 3 scripts

igordot

phenomenalist:Analysis Toolkit for PhenoCycler (CODEX) Data in R

A collection of tools for cleaning, clustering, and plotting PhenoCycler (CODEX) data.

Maintained by Igor Dolgalev. Last updated 1 years ago.

codex phenocycler

3 stars 2.18 score 1 scripts

welch-lab

SiNMFiD:Supervised iNMF informed Deconvolution

A package for completing cell type deconvolution on bulk spatial transcriptomic data utilizing multiple reference scRNA-seq datasets.

Maintained by Joshua Sodicoff. Last updated 1 years ago.

2.00 score 1 scripts

j-mitchel

scITD:Single-Cell Interpretable Tensor Decomposition

Single-cell Interpretable Tensor Decomposition (scITD) employs the Tucker tensor decomposition to extract multicell-type gene expression patterns that vary across donors/individuals. This tool is geared for use with single-cell RNA-sequencing datasets consisting of many source donors. The method has a wide range of potential applications, including the study of inter-individual variation at the population-level, patient sub-grouping/stratification, and the analysis of sample-level batch effects. Each "multicellular process" that is extracted consists of (A) a multi cell type gene loadings matrix and (B) a corresponding donor scores vector indicating the level at which the corresponding loadings matrix is expressed in each donor. Additional methods are implemented to aid in selecting an appropriate number of factors and to evaluate stability of the decomposition. Additional tools are provided for downstream analysis, including integration of gene set enrichment analysis and ligand-receptor analysis. Tucker, L.R. (1966) <doi:10.1007/BF02289464>. Unkel, S., Hannachi, A., Trendafilov, N. T., & Jolliffe, I. T. (2011) <doi:10.1007/s13253-011-0055-9>. Zhou, G., & Cichocki, A. (2012) <doi:10.2478/v10175-012-0051-4>.

Maintained by Jonathan Mitchel. Last updated 2 years ago.

cpp

1.98 score 19 scripts

tonisusin

coda4microbiome:Compositional Data Analysis for Microbiome Studies

Functions for microbiome data analysis that take into account its compositional nature. Performs variable selection through penalized regression for both, cross-sectional and longitudinal studies, and for binary and continuous outcomes.

Maintained by Toni Susin. Last updated 9 months ago.

2 stars 1.60 score 20 scripts

cran

sephora:Statistical Estimation of Phenological Parameters

Provides functions and methods for estimating phenological dates (green up, start of a season, maturity, senescence, end of a season and dormancy) from (nearly) periodic Earth Observation time series. These dates are critical points of some derivatives of an idealized curve which, in turn, is obtained through a functional principal component analysis-based regression model. Some of the methods implemented here are based on T. Krivobokova, P. Serra and F. Rosales (2022) <https://www.sciencedirect.com/science/article/pii/S0167947322000998>. Methods for handling and plotting Earth observation time series are also provided.

Maintained by Inder Tecuapetla-Gómez. Last updated 1 years ago.

1.00 score